梁琛 李伟 邵晓平 王意雯 唐惠.硫辛酸对卵巢切除小鼠学习记忆的影响[J].现代生物医学进展英文版,2016,16(29):5636-5639. |
硫辛酸对卵巢切除小鼠学习记忆的影响 |
Effect of Lipoic Acid on Learning and Memory Capacity of Ovariectomized Mice |
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DOI: |
中文关键词: 卵巢切除 硫辛酸 学习记忆 eNOS |
英文关键词: Ovariectomized Lipoic acid Learning and memory eNOS |
基金项目:江苏省自然科学基金项目(BK20150218) |
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中文摘要: |
目的:探讨硫辛酸(Lipoic acid,LA)对卵巢切除(ovariectomized,OVX)小鼠学习记忆的影响。方法:2月龄雌性昆明小鼠随机
分为4 组:假手术(Sham)组、OVX组、OVX+LA 组、OVX+LA+ 左旋硝基精氨酸甲酯(L-NAME)组。建立卵巢切除模型并给药,
Morris水迷宫检测各组小鼠学习记忆能力。结果:前4 天定位巡航实验中,OVX组与Sham组相比,逃避潜伏期明显延长(P<0.
05),第5 天撤除平台后,OVX组在目的象限游泳距离及时间百分比较Sham 组明显缩短(P<0.05);OVX+LA 组与OVX 组相比,
逃避潜伏期明显缩短(P<0.05),撤除平台后,在目的象限游泳距离及时间百分比明显延长(P<0.05);OVX+LA+L-NAME 组与
Sham 组相比,逃避潜伏期明显延长(P<0.05),撤除平台后,在目的象限游泳距离及时间百分比明显缩短(P<0.05)。结论:OVX损
伤小鼠的学习记忆,LA 可改善这种损伤,内皮型一氧化氮合酶(eNOS)抑制剂L-NAME 可削弱LA 的这种保护作用。 |
英文摘要: |
Objective:To investigate the influence of Lipoic acid (LA) on learning and memory abilites of Ovariectomize (OVX)
mice.Methods:2-month-old female Kunming mice (22-25 g) mice were assigned randomly to a shamoperated (Sham) group, ovariectomized
(OVX) group, ovariectomized+Lipoic acid (LA) group, and ovariectomized+ LA+L-NAME group. Morris water maze was used
to test the learning and memory abilities of mice.Results:Compared with the Shamgroup, in the OVX group, the average escape latency
detected by hidden platform trial were higher (P<0.05), the proportion of time and swim distance the mice spent searching for the platform
in the target quadrant were lower (P<0.05); as compared with the OVX group, LA shortened the escape latency (P<0.05), improve
the proportion of time and swimdistance(P<0.05); compared with the Sham group, in the OVX+LA+L-NAME group, the average escape
latency were higher (P<0.05), the proportion of time and swim distance the mice spent searching for the platform were lower (P<0.05).Conclusion:LA could improve the decreased of spatial learning and memory ability of mice which was induced by OVX. After an injection
of L-NAME, the above effects of LA were attenuated. |
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