徐光美 王俐丹 李嫄渊 吴淑燕 黄瑞△.沙门菌- 斑马鱼感染模型用于Th1/Th2 免疫应答的研究[J].现代生物医学进展英文版,2016,16(29):5606-5609. |
沙门菌- 斑马鱼感染模型用于Th1/Th2 免疫应答的研究 |
Th1/Th2 Immune Response in a Samonella-zebrafish Infection Model |
|
DOI: |
中文关键词: 鼠伤寒沙门菌 斑马鱼 Th1/Th2 免疫应答 |
英文关键词: Zebrafish Th1/Th2 immune response |
基金项目:国家自然科学基金面上项目(81471572) |
|
Hits: 711 |
Download times: 0 |
中文摘要: |
目的:通过构建斑马鱼成鱼感染模型,研究鼠伤寒沙门菌感染对机体Th1/Th2 免疫应答的影响。方法:用不同剂量细菌口饲
感染8 月龄的斑马鱼成鱼,绘制3 周生存率曲线。观察各剂量下对成鱼的感染情况,并用实时荧光定量聚合酶链式反应检测肝脏
Th1、Th2 型细胞相关基因和细胞因子的mRNA 转录水平,计算Th2/Th1 相对表达量比值。结果:用105 CFU感染2 周斑马鱼全
部存活,第15天开始出现死亡,且在3 周后死亡率达到50 %;感染后3 周解剖发现,肝脏、脾脏和肠道有明显红肿和糜烂;肝脏
Th1、Th2 型细胞相关基因和细胞因子mRNA转录水平明显向Th2 偏移。结论:用105 CFU 鼠伤寒沙门菌口饲感染斑马鱼成鱼构
建的模型,能反映机体感染和免疫功能变化,可用于研究体内Th1/Th2 免疫应答,为进一步研究鼠伤寒沙门菌感染与免疫机制提
供了很好的实验工具。 |
英文摘要: |
Objective:A Salmonella-zebrafish infection model was established to investigate the Th1/Th2 immune response initiated
by pathogenic bacteria.Methods:8-month-old zebrafish was infected by oral administration with different CFU. The survival curve
was drawn to show the life-or-death situation. The infection of adults' viscera was observed with the naked eye after that they are dissected
after 3 weeks. The expression levels of Th1, Th2 cell associated gene and cytokines mRNA in liver were measured by real-time quantitative
PCR and the ratio of corresponding cytokines for Th2/Th1 were also calculated.Results:All of adults were alive within 2 weeks
infected by 105 CFU infectious dose, while the death occurred on the 15th day. The death rate was 50 % three weeks later. With serious
red, swollen and erosion, the lesions of liver, spleen and intestine were significant, which were gained by means of dissecting the adults.
It was discovered that an obvious Th1/Th2 immune response was associated with 105 CFU infectious dose. Under this dose, a Th2 response
swift appeared evidently.Conclusion:It was feasible to establish a Salmonella-zebrafish infection model with 105 CFU oral administration
dose. The model was appropriate for reflecting infection and the immune function in the zebrafish adults, which could be
used to investigate Th1/Th2 immune response induced by pathogens. It was an available experimental implement to in-depth study the
mechanismof infection and immune induced by Salmonella typhimurium |
View Full Text
View/Add Comment Download reader |
Close |