| 眭鸿颖 颜志鹏 史彩霞 伍媚 贺艳.CDH1 基因启动子甲基化与宫颈癌临床病理类型的关系[J].现代生物医学进展英文版,2016,16(28):5486-5489. |
| CDH1 基因启动子甲基化与宫颈癌临床病理类型的关系 |
| The Relationship between CDH1 Gene Promoter Methylation and ClinicalPathological Types of Cervical Cancer |
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| DOI： |
| 中文关键词: 宫颈癌 CDH1 甲基化 启动子 |
| 英文关键词: Cervical cancer CDH1 Methylation Promoter |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨分析CDH1 基因启动子甲基化与宫颈癌临床病理类型的关系。方法：选取2012 年5 月~2015 年7 月我院105 例
宫颈癌患者为宫颈癌组,同时选取60 例正常宫颈组织为正常组，以甲基化特异性聚合酶链反应（MSP）检测CDH1 基因启动子
CpG岛甲基化状态及高危型HPV DNA状态，分析CDH1基因甲基化状态与高危型HPV DNA状态及临床病理参数的关系。结
果：宫颈癌组CDH1基因启动子甲基化阳性率为56.19%，明显高于正常组的6.67%，具有统计学差异（P<0.05）；宫颈癌组的高危
型HPV DNA阳性率为84.76%，明显高于正常组的20.00%，具有统计学差异（P<0.05）；高危型HPV DNA 与CDH1 基因启动子甲
基化的一致性分析结果具有统计学意义（P<0.05）；CDH1基因启动子甲基化率与患者的WHO 组织分化程度分级、FIGO 分期、组
织病理学分型、肿瘤大小有关，差异有统计学意义（P<0.05）。结论：宫颈癌CDH1 基因启动子甲基化与WHO 组织分化程度分级、
FIGO分期、组织病理学分型、肿瘤大小具有关联，并与高危型HPV DNA 阳性具有一致性，可以作为宫颈癌诊断和预后评估的参
考指标。 |
| 英文摘要: |
| Objective:To explore the relationship between CDH1 gene promoter methylation and clinical pathological types of
cervical cancer.Methods:105 cases of specimens from patients with cervical cancer (Cervical cancers group) and 60 cases of normal
cervical (Normal controls group) were selected, then methylation specific polymerase chain reaction (MSP) was used to detect the CDH1
gene start promoter CpG island methylation status of the samples and the high-risk HPV DNA status. After that we analyzed the
relationship between CDH1 gene methylation status and high-risk HPV DNA status, CDH1 gene methylation status and clinical
parameters of patients.Results:The positive rate of CDH1 gene promoter methylation in cervical cancer group was 56.19%, significantly
higher than that in the normal group (6.67%), with statistical difference (P<0.05). The positive rate of DNA HPV in cervical cancer group
was 84.76%, which was significantly higher than that in the normal group (20%), and the difference was statistically significant (P<0.05).
The consistency analysis results of DNA HPV and CDH1 gene promoter methylation were statistically significant (P<0.05). CDH1 gene
promoter methylation rate was significantly associated with the degree of WHO differentiation, FIGO stage, histological grade, tumor
size, which was statistically significant (P<0.05).Conclusion:The methylation of CDH1 gene promoter in cervical cancer is associated
with many clinical parameters (WHO differentiation grade, FIGO grades, Histological types, Tumor diameter), and is consistent with the
high risk type DNA HPV positive. It can be used as a reference index for the diagnosis and prognosis of cervical cancer. |
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