| 朱莽 谢江涛 樊欣鑫 方园 杨彦平 姜海涛.缺血预适应对小鼠脑缺血再灌注损伤模型血脑屏障的保护作用及机制[J].现代生物医学进展英文版,2016,16(28):5436-5439. |
| 缺血预适应对小鼠脑缺血再灌注损伤模型血脑屏障的保护作用及机制 |
| Protective Effect and Mechanismof Brain Ischemic Precondition on BloodBrain Barrier after Cerebral Ischemia Reperfusion Injury in Mice |
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| DOI： |
| 中文关键词: 脑缺血预适应 缺血再灌注 血脑屏障 HIF-1α VEGF |
| 英文关键词: BIP Ischemia reperfusion Blood Brain Barrier HIF-1α VEGF |
| 基金项目: |
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| 中文摘要: |
| 目的：通过研究缺血预适应对小鼠脑缺血再灌注损伤血脑屏障通透性的影响，探讨缺血预适应的脑保护作用及相关分子机
制。方法：取清洁健康成年小鼠72 只，随机分为脑缺血预适应组（brain ischemic precondition, BIP），脑缺血再灌注组（middle
cerebral artery occlusion and reperfusion, MCAO/R）和假手术组（sham group），每组均24 只，采用zealonga 线栓法栓塞小鼠大脑中
动脉建立BIP模型和MCAO/R 模型，通过氯化三苯基四氮唑（triphenyl tetrazolium chloride, TTC）染色计算脑梗死面积，改良神经
功能缺损评分（modified neurological severity scores, mNSS）对脑缺血再灌注神经损伤程度进行评估，测干- 湿重法以及伊文氏蓝
（Evans blue, EB）示踪结合脑组织EB 定量法评价血脑屏障（blood brain barrier, BBB）的损伤程度，采用免疫组化法检测各组脑组
织低氧诱导因子-α（HIF-1α）和血管内皮生长因子（VEGF）的表达。结果：与MCAO组相比，BIP 组显著降低缺血再灌注后mNSS
评分，缩小了梗死面积并减轻脑水肿，有效的保护BBB功能，BIP 组再灌注24 h时脑梗死灶周围皮质区HIF-1α及VEGF 的表达
均明显上调，差异有统计学意义（P＜0.05）。结论：BIP对小鼠脑缺血再灌注损伤模型BBB 有一定的保护作用，其机制可能与其诱
导HIF-1琢及VEGF的表达上调有关。 |
| 英文摘要: |
| Objective:To study the effect of brain ischemic precondition on blood brain barrier after cerebral ischemia reperfusion
injury in mice, and to expain the potential molecular mechanisms.Methods:Seventy-two clean and healthy mice were randomly
assigned in brain ischemic preconditioning (BIP) group, middle cerebral artery occlusion and reperfusion (MCAO/R) group and sham
group, 24 mice in each group. The BIP model and MCAO/R model were induced by Zealonga middle cerebral artery occlusiono
(MCAO) methods, the infarction volume was calculated by TTC stain, the neurological deficit scores were analyzed by mNSS, brain
water content was measured by Dry/Wet method, Evans Blue tracer combine brain tissue EB content was used to evaluate BBB
permeability, the expression of hypoxia-inducible factor-1α(HIF-1α) and vascular endothelial growth factor (VEGF) in each mice brain
tissue were detected by immunohistochemistry.Results:The expression of HIF-1αand VEGF in ischemic cortex periphery of BIP group
were significantly elevated at 24 h after reperfusion compared with sham group (P <0.05); Fortunately, BIP can significantly reduce
infarction volume and cerebral edema, effectively protect BBB dysfunction and reduce mNSS scores after ischemia reperfusion injury in
mice.Conclusion:BIP has protective effect on BBB dysfunction in the mice model of cerebral ischemia reperfusion injury, which might
be related to the increased expression of HIF-1αand VEGF. |
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