| 刘一诚 戴彭辰 李慧英 腾柱国 何成诗.共刺激因子B7-H3 在非小细胞肺癌中的表达研究[J].现代生物医学进展英文版,2016,16(27):5309-5312. |
| 共刺激因子B7-H3 在非小细胞肺癌中的表达研究 |
| Expression and Clinical Significance of co-stimulantory Molecule B7-H3 inNon-Small Cell Lung Cancer |
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| DOI： |
| 中文关键词: 非小细胞肺癌 B7-H3 免疫组织化学 |
| 英文关键词: Non-small cell lung cancer B7-H3 Immunohistochemistry |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨共刺激椅子B7-H3 在人不同非小细胞肺癌（NSCLC）细胞系和组织中的表达及其临床意义。方法：实时定量反转
录-聚合酶链式反应及蛋白印迹法检测B7-H3 在5 种人NSCLC 细胞系中的表达量，利用免疫组化法检测121 例NSCLC、33 例
癌旁组织及36 例良性病变组织中B7-H3 的表达量，并收集患者临床资料，分析B7-H3 与临床参数的关系。结果：5 个NSCLC 细
胞系中均见B7-H3 的表达，A549 及SPAC-1 中表达较H466、H1299 及H460 中表达较低；B7-H3 在NSCLC组织中高于癌旁组织
及良性病变组织（P<0.001）；分化程度高者平均IOD高于分化程度中、低的患者（P<0.05）。发生淋巴结转移和未发生淋巴结转移的
患者B7-H3 的平均IOD之间存在显著性差异（P＜0.05）。结论：B7-H3 在NSCLC组织中高表达，可能是疾病治疗或诊断的靶因
子。 |
| 英文摘要: |
| Objective:To investigate the expression and clinical significance of co-signal molecule B7-H3 in non-small cell lung
cancer (NSCLC) cell lines and tissues.Methods:B7-H3 expression level was evaluated by RT-PCR and western blot in five NSCLC cell
lines. Specimens from 121 participants were stained immunohistochemically. Staining was quantified using Image-Proplus software, and
integrated optical density was measured on each section.Results:The constitutive expression of B7-H3 in all five cell lines was found.
The expression of B7-H3 were higher than that in benign lesions and non-cancer tissues respectively (P<0.001). B7--H 3 expression in
well differentiated NSCLC was higher than that in not well differentiated one (P<0.05).There was a significant association between expression
of B7-H3 and lymph node metastasis.Conclusion:B7-H3 is overexpressed in NSCLC tissues and may be an important target for
diagnosis or the therapy of the disease. |
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