| 张崇友 张桂梅 汪丹丹 赵丽 杨微.Lin28A促进结肠癌细胞对5-FU 化疗敏感性的实验研究[J].现代生物医学进展英文版,2016,16(27):5246-5249. |
| Lin28A促进结肠癌细胞对5-FU 化疗敏感性的实验研究 |
| Experimental Study on Effect of Lin28A on the Sensitivity of Colon Cancer 5-FU Chemotherapy |
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| DOI： |
| 中文关键词: 结肠癌 Lin28A 化疗敏感性 let-7 |
| 英文关键词: Colon cancer Lin28A Chemosensitivity Let-7 |
| 基金项目:黑龙江省教育厅科学技术研究项目（12531296）；黑龙江省自然科学基金面上项目（H2015008） |
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| 中文摘要: |
| 目的：探讨Lin28A 对结肠癌5- 氟尿嘧啶（5-FU）化疗敏感性的影响及其分子机制。方法：免疫组化方法检测人结肠癌和正
常粘膜组织中Lin28A 蛋白表达情况；荧光素酶法检测HCT116 细胞活性；实时荧光定量PCR方法检测HCT116 细胞中Let-7 表
达水平。结果：73.3%人结肠癌患者Lin28A 蛋白表达上调；过表达Lin28A 组与对照组相比，5-FU 处理后细胞活性显著降低
（P60ug<0.01，P80ug<0.01），Let-7 表达显著降低；过表达Let-7 组与对照组相比，5-FU治疗后细胞活性显著降低（P40ug<0.05，P60ug<0.01，
P80ug<0.01）。结论：Lin28A 可增强肿瘤细胞化疗敏感性，而且这一效应不依赖Let-7。 |
| 英文摘要: |
| Objective:To investigate the effect of Lin28A on the sensitivity of colon cancer 5-FU chemotherapy and its molecular
mechanism.Methods:The expression of Lin28A in human colon carcinoma and normal mucosa was detected by immunohistochemistry
method. HCT116 cell activity was detected by luciferase assay. The Let-7 expression in HCT116 cells was detected by real-time quantitative
PCR method.Results:Lin28A is up-regulated in 73.3 %of colon cancer patients. After 5-FU treatment, the cell activity was significantly
decreased(P60ug<0.01,P80ug<0.01) in Lin28A over-expressing cells compared to control cells. The Let-7 expression was significantly
decreased at the same time. Compared with the control group, the cell activity after 5-FU treatment in Let-7 overexpression group was
significantly lower than that in the control group (P40ug<0.05, P60ug<0.01, P80 ug<0.01).Conclusion:The effect of Lin28A increasing
chemosensitivity of cancer cells is in a Let-7 independent manner. |
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