| 胡小平 王志维△ 吴红兵 胡锐 程良昊.主动脉夹层血管壁中氧化应激蛋白NOX1 表达及意义[J].现代生物医学进展英文版,2016,16(27):5206-5209. |
| 主动脉夹层血管壁中氧化应激蛋白NOX1 表达及意义 |
| Expression and Significance of NADPH Oxidase-1 in the Vessel Wall ofAortic Dissection |
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| DOI： |
| 中文关键词: 主动脉夹层 NADPH氧化酶-1 弹性纤维 病因 |
| 英文关键词: Aortic dissection NADPH oxidase-1 Elastic fibers Etiology |
| 基金项目:国家自然科学基金面上项目（81570428）；中央高校基本科研业务费专项资金项目（2042014kf0117）；
湖北省自然科学基金面上项目（2015CFB169，2013CKB031） |
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| 中文摘要: |
| 目的：探讨主动脉夹层(aortic dissection，AD)血管组织中氧化应激蛋白NOX1 的表达及意义。方法：收集2014年~2015年在
武汉大学人民医院AD 患者升主动脉血管组织标本(AD组)及多器官捐献患者升主动脉血管组织标本(对照组)各12 例。采用维多
利亚蓝染色观察主动脉中膜弹性纤维形态结构；应用SP 免疫组织化学法及Western blot 法对标本组织中的NOX1 进行检测分
析。结果：AD 组主动脉中膜弹性纤维形态和排列不规则、破碎、丢失，结构紊乱。免疫组织化学显示NOX1 阳性表达见于主动脉壁
平滑肌细胞胞质中，AD 组与对照组比较，NOX1表达明显升高。Western blot 蛋白印迹示AD组NOX1表达明显增高，差异有统
计学意义(P<0.05)。结论：NOX1 在AD 主动脉中膜中表达上调，可能在AD的发生中发挥作用。 |
| 英文摘要: |
| Objective:To study the differential expression of NADPH oxidase-1 (NOX1) between the aorta specimens from the
aortic dissection (AD) and the normal controls.Methods:Ascending aorta specimens were taken from patients of acute Stanford A dissection
and multi-organ donors, which divided into AD group (n=12) and the control group (n=12). Victoria blue staining was used for
identifying the pathological changes of elastic fibers in the aortic media. SP immunohistochemistry and Western blot were used to observe
the differential expression of NOX1 between the AD group and the control group.Results:The morphology and arrangement of elastic
fibers in the aortic media were anomalous, and the disappearance, loss and chaos of elastic fibers were also found in AD group. Immunohistochemistry
presented that the expression of NOX1 was higher in the AD group than control group. Western blot also presented that
the expression of NOX1 was higher in the AD group with statistical significance (P<0.05) than control group.Conclusion:Up-regulation
of NOX1 in the aortic media of AD suggests that NOX1 may an active role in the progress of AD. |
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