| 陈家宽 王文辰 杨波 夏彦民 苏向妮 周琳 葛忠虎 杨峰 姜涛.NDRG2 和ZC3H12D在大鼠急性肺损伤中的表达及其相关性的研究[J].现代生物医学进展英文版,2016,16(25):4834-4839. |
| NDRG2 和ZC3H12D在大鼠急性肺损伤中的表达及其相关性的研究 |
| The Expression and Relationship between NDRG2 and ZC3H12D in LungTissues of Rats with Acute Lung Injury by LPS |
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| DOI： |
| 中文关键词: 败血症 急性肺损伤 N-myc 下游调节基因-2 具有CCCH锌指结构域的蛋白质12d 核转录因子-κBp65 |
| 英文关键词: Sepsis Acute lung injury NDRG2 ZC3H12D NF-κBp65 |
| 基金项目:陕西省自然科学基础研究计划项目(2014JM2-8144) |
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| 中文摘要: |
| 目的：通过建立LPS诱导的大鼠急性肺损伤模型，观察大鼠肺组织中N-myc 下游调节基因2(NDRG2)和具有CCCH锌指结
构域的蛋白质12d(ZC3H12D)表达水平的变化来讨论二者相互作用的机制。方法：40 只健康成年雄性SD 大鼠随机分成对照组、
LPS 注射60 min、120 min 和180 min 组，获取各组大鼠肺组织，行湿干重比值(W/D)检测、ELISA检测、病理(HE)检测确定肺损伤
模型是否建立成功；免疫组化、RT-PCR 和Western-blot 对NDRG2 和ZC3H12D 在mRNA 水平和蛋白水平进行检测。结果：各
LPS损伤组与对照组相比，W/D 比值显著升高，180 min 组比值最高，为5.68± 0.52 (P<0.05) ，血清和肺匀浆中TNFα和IL-1β含量明显增加（P<0.05）；损伤组肺泡壁增宽，炎细胞浸润明显；肺组织NDRG2 和ZC3H12D在mRNA 水平和蛋白水平的表达都呈
相降低后回升的趋势，180 min 组NDRG2 和ZC3H12D,蛋白含量分别为对照组的0.37 和0.44 倍(P<0.05)。结论：LPS 诱导的大鼠
急性肺损伤可同时下调肺组织中NDRG2 和ZC3H12D的表达，并且NDRG2 和ZC3H12D 具有潜在的相关性，提示两者可能存
在互相调节的机制，并通过调节NF-κB 通路对肺损伤的大鼠起保护作用。 |
| 英文摘要: |
| Objective:To investigate the changes of the expression levels of zc3h12d gene and ndrg2 gene in lung tissue of rats
with lipopolysaccharide-induced acute lung injury.Methods:40 healthy male Sprague-Dawley (SD) rats were randomized into 5 groups:
control group, LPS 60min group, LPS 120min group and LPS 180min group. Control group and LPS groups were treated with tail intravenous
injection of saline or LPS(6 mg/kg) to establish ALI model. Then lung tissues were examined through wet／dry (W/D) ratio and
were observed histologic change through hematoxylin-eosin (HE) dyeing; TNFα, IL-1βlevels in serum and lung tissue was measured by
enzyme-linked immunosorbent assay (ELISA); The dynamic expression of zc3h12d gene and ndrg2 gene in lung tissue were examined by
qRT-PCR, immunohistochemistry and Western blot.Results:Compared with the control group, the lung wet/dry (W/D) ratio of LPS
groups was significant increased, 180 min group is 5.68± 0.52 (P<0.05); Lung tissues emerged inflammation changes; TNFα, IL-1βlevels
in serum and lung tissue significant increased (P<0.05). The expression of NDRG2 and ZC3H12D mRNA significantly decreased
(P<0.05); The expression of NDRG2 and ZC3H12D significant decreased (P<0.05), the expression of phosphorylated NF-κBp65 significant
increased (P<0.05).Conclusion:Acute lung injury induced by LPS in rats and downregulate the expression in the lung tissue of
NDRG2 and ZC3H12D, and NDRG2 and ZC3H12D has potential relevance, suggests that the two may have the mutual regulation
mechanism, and by adjusting the NF kappa B pathway protective effect on lung injury in rats |
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