| 戴彭辰 刘一诚 何成诗 黄青松 李慧英 王超群.波形蛋白在非小细胞肺癌组织中的表达及其临床意义[J].现代生物医学进展英文版,2016,16(23):4481-4483. |
| 波形蛋白在非小细胞肺癌组织中的表达及其临床意义 |
| Expression of Vimentin in Non-Small Cell Lung Cancer Tissue and ItsClinical Significance |
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| DOI： |
| 中文关键词: 非小细胞肺癌 波形蛋白 免疫组织化学 |
| 英文关键词: NSCLC Vimentin Immunohistochemistry |
| 基金项目: |
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| 中文摘要: |
| 目的：探究波形蛋白在非小细胞肺癌（NSCLC）组织中的表达及其与肺癌浸润转移的相关性。方法：收集2012 年6 月-2014
年6 月我院手术切除的NSCLC 癌组织标本150 例及癌旁正常组织（距肿瘤＞5 cm）79 例，提取两组的RNA，采用实时荧光定量
聚合酶链反应（RT-PCR）检测波形蛋白mRNA表达水平，免疫组化法检测波形蛋白的蛋白表达，分析波形蛋白表达水平与淋巴结
转移、TNM分期的相关性。结果：波形蛋白mRNA在NSCLC 癌组织中的表达明显高于癌旁正常组织（P＜0.05）。NSCLC 癌组织
中波形蛋白mRNA表达水平的上调与淋巴结转移及TNM分期（P＜0.05）相关。结论：波形蛋白在NSCLC 患者中表达异常升高，
与NSCLC 的发生和浸润转移密切相关。 |
| 英文摘要: |
| Objective:To investigate the expression of vimentin in non-small cell lung cancer (NSCLC) tissues and the relationship
with invasion and metastasis of lung cancer.Methods:Collected 150 cases of NSCLC carcinoma tissues and 79 cases of adjacent
normal tissues (>5cm away from carcinoma tissues) from the resection underwent in our hospital from June 2012 to June 2014, then extracted
RNA of two groups, detected the expression of vimentin mRNA by real time-polymerase chain reaction (RT-PCR) method, and
detected the expression of vimentin protein by immunohistochemical method, finally, analyzed the relationship between the expression of
vimentin protein and the lymph node metastasis and TNMstage.Results:The expression of vimentin mRNA expression in NSCLC carcinoma
tissues was significantly higher than adjacent normal tissues(P<0.05). The up-regulation expression of vimentin mRNA was related
with lymph node metastasis and TNMstage (P<0.05).Conclusion:The expression of vimentin is up-regulation in NSCLC,which is closely
related to the occurrence and invasion of NSCLC. |
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