| 孙凤军 史惠卿 冯伟 孙艺璇 夏培元△.亚抑菌浓度头孢他啶对大肠埃希菌生物膜形成的影响与其耐药性相关性研究[J].现代生物医学进展英文版,2016,16(20):3839-3844. |
| 亚抑菌浓度头孢他啶对大肠埃希菌生物膜形成的影响与其耐药性相关性研究 |
| The Correlation Analysis between the Effect of Sub-minimal InhibitoryConcentration Ceftazidime on the BiofilmFormation of andIts Bacterial Resistance* |
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| DOI： |
| 中文关键词: 大肠埃希菌 亚抑菌浓度 头孢他啶 生物膜 耐药性 |
| 英文关键词: Sub-minimal inhibitory concentration Ceftazidime Biofilm Resistance |
| 基金项目:国家自然科学基金项目(81373451) |
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| 中文摘要: |
| 目的：探讨亚抑菌浓度头孢他啶对大肠埃希菌生物膜形成的影响与细菌耐药性、超广谱β- 内酰胺酶(ESBLs)产生及ESBLs
基因分型的相关性，为临床生物膜感染的治疗和抗生素的合理使用提供理论依据。方法：大肠埃希菌最低抑菌浓度(MIC)检测采
用琼脂平板倍比稀释法，超广谱β- 内酰胺酶(ESBLs)表型确证实验采用双纸片协同法，大肠埃希菌ESBLs 基因检测采用PCR 扩
增，生物膜形成能力检测采用96 孔板结晶紫染色法。结果：50 株大肠埃希菌临床株对青霉素类、氟喹诺酮类、头孢哌酮及复方新
诺明具有较高的耐药性，而对阿米卡星、哌拉西林/他唑巴坦敏感性较高。所有菌株均对碳青霉烯类抗菌药物敏感。31株大肠埃
希菌为ESBLs阳性菌株。CTX-M、TEM、OXA、SHV和VEB基因阳性率分别为93.5 %、83.9 %、19.4 %、16.1 %和3.2 %。亚-MIC
头孢他啶对9 株（18.0%）大肠埃希菌生物膜形成具有抑制作用。亚-MIC 头孢他啶对大肠埃希菌生物膜形成的影响与细菌耐药性
和ESBLs 均无相关性（P>0.05）。结论：亚-MIC 头孢他啶对大肠埃希菌生物膜形成的调控作用与细菌耐药性、产ESBLs及ESBLs
基因分型均无相关性。 |
| 英文摘要: |
| Objective:To study the correlation between the effect of sub-minimal inhibitory concentration (sub-MIC) ceftazidime
on the biofilm formation of Escherichia coli and its bacterial resistance and ESBLs, and provide a theoretical basis for the clinical
treatment of biofilm infection and rational use of antibiotics.Methods:The minimal inhibitory concentration was determined by the agar
double dilution method. Extended-spectrum beta-lactamases (ESBLs) phenotypic confirmatory test was detected by double-disk synergy
method. ESBLs genes were detected by PCR amplification. The biofilm formation was assayed using the 96-well crystal violet staining
method.Results:50 E.coli isolates were high resistant to penicillins, fluoroquinolones, cefoperazone and trimethoprim-sulfamethoxazole,
whereas they were high sensitive to amikacin and piperacillin/tazobactam. All the strains were sensitive to carbapenem. A total of 31
isolates were ESBLs-positive strains. The positive rates of CTX-M, TEM, OXA, SHV and VEB genes were 93.5 %, 83.9 %, 19.4 %,
16.1%and 3.2 %, respectively. Sub-MIC ceftazidime could inhibit the biofilmformation of 9 (18.0%) E.coli isolates. No correlation was
found between the effect of sub-MIC ceftazidime on the biofilm formation of and bacterial resistance and ESBLs (P>0.05).Conclusion:No correlation was found between the regulation of sub-MIC ceftazidime on the biofilm formation of E.coli and bacterial
resistance, ESBLs production and ESBLs genotypes. |
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