史滢深 汪汇 郭荣 侯强 韩童 江华1.软骨膜对兔耳颗粒软骨定形移植物组织学及生物力学特性的影响[J].现代生物医学进展英文版,2016,16(20):3831-3834. |
软骨膜对兔耳颗粒软骨定形移植物组织学及生物力学特性的影响 |
Effect of Perichondriumon the Histological and Biomechanical Properties ofDiced Cartilage Graft with CustomShape |
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DOI: |
中文关键词: 颗粒软骨 移植物 软骨膜 生物力学 |
英文关键词: Diced cartilage Graft Perichondrium Biomechanics |
基金项目:上海长征医院青年启动基金(2013CZQN03) |
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中文摘要: |
目的:通过高密度聚乙烯模具构建兔耳颗粒软骨定形移植物,并观察软骨膜对其组织学及生物力学特性的影响。方法:8 只
雄性新西兰大白兔,取一侧耳软骨后制备定形颗粒软骨移植物,分为保留软骨膜及去除软骨膜组,埋植于背部皮下,术后1 月、2
月、3 月取材进行组织学及生物力学检测。结果:无论保留软骨膜与否,颗粒软骨均可长为具备一定形状和机械强度的均一整体,
保留软骨膜组中可观察到Ⅱ型胶原及胶原纤维酸性蛋白(GFAP)免疫组化阳性染色的新生软骨组织,其破坏应力和弹性模量均
高于去除软骨膜组(P<0.05)。结论:高密度聚乙烯模具可以很好地完成颗粒软骨定形移植物的构建,保留软骨膜的颗粒软骨具有
更好的组织学活性,以及更佳的生物力学特性。 |
英文摘要: |
Objective:To observe the effect of perichondrium on its histological and biomechanical properties by constructing
diced cartilage grafts with custom shape with high-density porous polyethylene mould wrapped.Methods:Unilateral auricular cartilage
were harvested from 8 rabbits and diced to 1mm cube pieces, divided into 2 groups according to the preservation of perichondrium. The
autologous diced cartilage grafts were implanted into the dorsal subcutaneous layer, and took out for histological and biomechanical tests
at 1, 2 and 3 months respectively after the implantation.Results:The diced cartilage grafts grew into one-piece masses with certain
mechanical strength in both two groups. New generative cartilage tissue with positive immunohistochemistry staining of type Ⅱ collagen
and glial fibrillary acidic protein (GFAP) was observed in grafts with perichondrium preserved, and their failure stress and modulus of
elasticity were higher than those with perichondrium removed (P<0.05).Conclusion:Diced cartilage graft with custom shape can be
constructed with high-density porous polyethylene as mould, and the preservation of perichondrium can improve its histological and
biomechanical properties. |
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