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目的：制备担载血管生长因子（VEGF）的乳液法静电纺丝纤维膜，对其开展一系列表征，从而研究其血管再生的潜能。方法：通过W/O 乳液法制备担载VEGF的静电纺丝纤维膜，并对其形态、力学性质进行表征。用VEGF ELISA 分析方法对其体外释放动力学进行研究。运用CCK-8 法检测乳液法静电纺丝纤维膜中VEGF的活性变化。结果：乳液法静电纺丝纤维膜呈现连通的三维网状结构，平均直径为1 滋m，模拟了细胞外基质（ECM），最大拉伸应力为3.03± 0.66 MPa，具有良好的抗拉伸能力，能够支持细胞的生长。乳液法纤维膜中VEGF在体外累积释放了14天，总释放量超过20000 pg，达到血管再生的有效浓度。CCK-8 结果显示，乳液法纤维膜中的VEGF仍然保持较高的蛋白活性。结论：担载VEGF的乳液法静电纺丝纤维膜能够缓释出活性的蛋白，具有血管再生的潜能。

英文摘要:

Objective:To study the potential of angiogenesis by preparing emulsion method electrospun fibers loading vascular endothelial growth factor (VEGF) and carrying out a series of characterization.Methods:Electrospun fibers loading VEGF were prepared by W/O emulsion method and were characterized in morphology and mechanical properties. The release kinetic of VEGF in electrospun fibers was detected by ELISA method in vitro. By CCK-8 method, the changes of active situation of VEGF in electrospun fibers were investigated.Results:Emulsion method electrospun fibers were connected by three-dimensional network structure with average diameter 1 滋m, which simulated the extracellular matrix (ECM) and they had good tensile strength which could support cell growth, their maximum tensile stress was 3.03± 0.66 MPa. VEGF in emulsion method fibers had cumulative release of 14 days, the total amount was over 20000 pg which could reach the effective concentration of angiogenesis. The result of CCK-8 tests showed that VEGF loaded in emulsion method fibers still maintained high activity.Conclusion:Emulsion method electrospun fibers loading VEGF had a sustained-release of active protein, which had the potential of angiogenesis.

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