| 苗锐 赵耀 杨荣军 高兴春 郭娜.紫草素对人脑胶质瘤U251 细胞增殖和凋亡的影响[J].现代生物医学进展英文版,2016,16(19):3646-3649. |
| 紫草素对人脑胶质瘤U251 细胞增殖和凋亡的影响 |
| Study on U251 Glioma Cell Proliferation and Apoptosis induced by Shikonin |
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| DOI： |
| 中文关键词: 紫草素 胶质瘤 细胞凋亡 Bax Bcl-2 |
| 英文关键词: Shikonin Glioma Apoptosis Bax Bcl-2 |
| 基金项目:陕西省卫生科研项目（2014D21） |
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| 中文摘要: |
| 目的：观察紫草素对体外培养的人脑胶质瘤U251 细胞的增殖和凋亡的影响。方法：应用MTT 法和AnnexinV-FITC/PI双染
流式细胞术分别检测2.5、5、10 uM/L的紫草素对U251 细胞的体外抑杀作用以及凋亡诱导作用，进一步应用Western blot 方法检
测紫草素对凋亡相关蛋白Bcl-2 及Bax 表达水平的影响。结果：紫草素对人U251 胶质瘤细胞具有明显的增殖抑制作用，且呈一
定的剂量依赖性和时间依赖性。紫草素可明显上调U251 细胞Bax 的表达，下调Bcl-2 的表达，与对照组相比存在显著性差异
（P<0.05）。结论：紫草素对人胶质瘤U251细胞具有明显的抑制增殖和促进凋亡作用。 |
| 英文摘要: |
| Objective:To study the effect of shikonin on the proliferation and apoptosis of U251 glioma cells in vitro.Methods:MTT assay and AnnexinV-FITC/PI double staining flow cytometry were used to test the cell proliferation and apoptosis induced by 2.5,
5, 10 uM/L shikonin on U251 cells. Western blot was used to detect apoptosis-related protein Bcl-2 and Bax expression levels.Results:Shikonin exhibited prominent inhibition effect on U251 cells proliferation in a dose-dependent and time-dependent manner. Shikonin significantly
upregulated the expression of Bax and downregulated the expression of Bcl-2.Conclusion:Shikonin could significantly inhibit
the proliferation and promote apoptosis on human glioma U251 cells. |
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