| 付亚康 李锡忠 夏晓 樊先茂 刘耀文 翁杰.羟基磷灰石Zeta电位对其蛋白吸附性能的影响[J].现代生物医学进展英文版,2016,16(19):3610-3613. |
| 羟基磷灰石Zeta电位对其蛋白吸附性能的影响 |
| Effect of Zeta Potential of Hydroxyapatite on Protein Adsorption |
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| DOI： |
| 中文关键词: 羟基磷灰石 Zeta 电位 蛋白吸附 静电作用 竞争吸附 |
| 英文关键词: Hydroxyapatite Zeta potential Protein adsorption Electrostatic interaction Competitive adsorption |
| 基金项目:国家重点基础研究发展计划(2012CB933600)；国家自然科学基金项目(51172188)；
四川省高校科研创新团队建设计划项目(14TD0050) |
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| 中文摘要: |
| 目的：为了寻找羟基磷灰石（HA）颗粒的蛋白吸附性能与其Zeta 电位之间的关系，为研发具有优秀骨修复能力的HA材料
提供理论依据。方法：本文采用水热反应法，合成了形状规则的带状HA 颗粒，并采用SEM、BET、XRD 对其进行表征。检测了HA
颗粒在不同pH、PO4
3-浓度中的Zeta 电位；选择小牛血清白蛋白（BSA）、溶菌酶（LYS）两种蛋白作为模型蛋白，测试了HA颗粒在
不同pH、PO4
3-浓度环境中对BSA或LYS的吸附量。结果：随着吸附体系的pH值逐渐增大，HA颗粒表面负电性迅速增加，HA
颗粒对BSA的吸附能力逐渐减弱，对LYS 的吸附能力逐渐增强。随着吸附体系中PO4
3-浓度逐渐增大，HA 颗粒表面负电性呈先
增大后减小的变化趋势，对BSA、LYS 的吸附性能均逐渐减弱。结论：pH 和PO4
3-浓度的变化，都能引起HA 颗粒Zeta 电位的变
化，前者引起静电作用类型、强度的变化而改变HA 蛋白吸附性能；后者引入竞争吸附，蛋白吸附量随竞争离子浓度增多而逐渐减
少。 |
| 英文摘要: |
| Objective:In order to research the correlation between surface voltage and protein adsorption property of hydroxyapatite
(HA), provide valuable evidences for developing HA materials with excellent ability of bone repair.Methods:HA particles were hydrothermally
synthesized and characterized by SEM、BET and XRD. The Zeta potentials of HA in different pH or concentration of PO4
3-
were examined. The protein adsorption amounts of negatively charged bovine serum albumin (BSA) and positively charged lysozyme
(LYS) on HA in different pH or concentration of PO4
3- were examined.Results:The results indicated that as the increasing of pH in the
adsorption system, the negative charge on HA surface also increased quickly and BSA adsorption ability slowly weakened, but the LYS
adsorption ability gradually intensified. It was also found out that as the increasing of PO4
3- concentration in the adsorption system, the
negative charge on HA surface showed a trend of first increasing and then decreasing, and the protein adsorption abilities of BSA and
LYS both weakened gradually.Conclusion:The changes of pH and PO4
3- concentration can both regulate the Zeta potential of HA particles,
the former is caused by the changes of the type and intensity of the electrostatic interaction, which regulate the protein adsorption
capability of HA; the latter is due to the introducing of competition adsorption, and the protein adsorption amount decreases with the increasing
of the competition ion concentration. |
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