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目的：研究白细胞介素-6（IL-6）、白细胞介素-1beta（IL-1beta）及肿瘤坏死因子-alpha（TNF-alpha）与大鼠骨质疏松形成的关系，为研究细胞因子与骨质疏松之间的相关作用机制提供参考。方法：选择2015 年1 月至2015 年11 月我院采购的90 只雌性大鼠作为研究对象，按照数字随机法将大鼠分成观察组（n=45）以及对照组（n=45）。观察组制成骨质疏松模型，对照组不作处理，对比两组局部骨密度，成骨细胞,骨小梁以及破骨细胞在视野面积中的比例，骨组织相关细胞因子水平，分析IL-6、IL-1beta以及TNF-alpha水平与大鼠骨质疏松的相关性。结果：观察组椎体骨密度（BD）和椎间盘BD 以及小关节BD 均明显低于对照组，差异均有统计学意义（P＜ 0.05）；观察组骨小梁和成骨细胞在视野面积中的比例明显低于对照组，而破骨细胞在视野面积中的比例明显高于对照组，差异均有统计学意义（P＜0.05）；观察组IL-6 和IL-1茁以及TNF-琢均明显高于对照组，差异有统计学意义（P＜0.05）。IL-6、IL-1茁以及 TNF-琢水平与大鼠椎体BD、椎间盘BD 以及小关节BD 均呈明显负相关。结论：去卵巢大鼠的细胞因子与其骨质疏松具有紧密联系，表现在IL-6、IL-1茁以及TNF-琢水平与大鼠椎体BD、椎间盘BD 以及小关节BD 均呈明显负相关。

英文摘要:

Objective:To study the correlation between IL-6, IL-1beta,TNF-alpha and the formation of osteoporosis in rats, to provide reference for the related mechanism of cytokines and osteoporosis.Methods:Selected 90 female rats which were purchased by our hospital from January 2015 to November 2015 as the research object, and were randomly divided into observation group (n=45) and control group (n=45). Osteoporosis model was made in the observation group, control group was not treated, compared the ratio of local bone mineral density, osteoblasts, bone trabecula and osteoclasts of two groups in visual area, the cytokine levels in bone tissuewas, and analyzed the correlation between IL-6, IL-1beta, TNF-alpha and osteoporosis in rats.Results:The vertebral bone density (BD), intervertebral disc BD and small joint BD of observation group were significantly lower than that of the control group, the differences were statistically significant (P<0.05); the ratio of bone trabecula and osteoblasts in the visual area were significantly lower than that of the control group, while the ratio of osteoclasts in the visual area was significantly higher than that of the control group, the differences were statistically significant (P<0.05); the IL-6,IL-1beta and TNF-alpha of observation group were significantly higher than that of the control group, the differences were statistically significant (P<0.05). The IL-6, IL-1beta ,TNF-alpha were negatively correlated with vertebral BD, intervertebral disc BD and the small joint BD of the rat vertebral body.Conclusion:The cytokines is closely related to osteoporosis in ovariectomized rats, performance in IL-6, IL-1beta, TNF-alpha are negatively correlated with vertebral BD, intervertebral disc BD and small joint BD of the rat vertebral body.

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