| 李会平 尤新国 魏露 樊姝彤 刘晓影 陈丽梅 潘智芳△ 冯卫国.锌指蛋白185 在小鼠睾丸支持细胞中的表达与定位[J].现代生物医学进展英文版,2016,16(18):3439-3442. |
| 锌指蛋白185 在小鼠睾丸支持细胞中的表达与定位 |
| The Expression and Localization of Zinc Finger Protein 185 in the SertoliCells of the Mouse Testis |
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| DOI： |
| 中文关键词: 锌指蛋白（ZNF）185 支持细胞 表达 定位 精子发生 |
| 英文关键词: ZNF185 Sertoli cell Expression Localization Spermatogenesis |
| 基金项目:山东省科技发展计划项目（2015GSF118178）；山东省自然科学基金项目（ZR2013CM032）；
山东省卫计委科技基金项目（201411）；潍坊医学院科技创新基金项目（K1302001） |
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| 中文摘要: |
| 目的：检测锌指蛋白185 在小鼠睾丸支持细胞中的表达，探讨其与精子发生的关系。方法：提取睾丸和支持细胞总RNA，经
半定量RT-PCR 法检测ZNF185 的转录水平；提取睾丸和支持细胞的蛋白质，利用Western blot 分析ZNF185 的表达量；制备支持
细胞爬片，采用免疫荧光技术检测ZNF185 的定位。结果：（1）半定量RT-PCR 结果显示：在睾丸支持细胞中扩增出ZNF185 基因
条带。（2）Western blot 结果显示：ZNF185 在支持细胞中的表达量显著低于在睾丸组织中的表达量。（3）免疫荧光结果显示：
ZNF185主要定位于支持细胞胞质中。结论：ZNF185可能参与支持细胞结构与功能的调控，进而影响精子发生过程。 |
| 英文摘要: |
| Objective:To detect the expression of Zinc finger protein 185 in the sertoli cells of the mouse testis and to investigate
the relationships between ZNF185 and spermatogenesis.Methods:The total RNA was extracted from the testis and sertoli cells cells, the
transcription level of ZNF185 was detected by using semi-quantitative RT-PCR assay; proteins were extracted from the testis and sertoli
cells, the expression level of ZNF185 was analyzed by Western blot; the coverglass-cultured cells were prepared, the positioning of
ZNF185 was detected by immunofluorescence technique.Results:(1) The semi-quantitative RT-PCR showed that ZNF185 gene was
amplified in the sertoli cells of the testis. (2) The result of Western blot showed that the expression of ZNF185 in the sertoli cells was
significantly lower than that in the testis. (3) Immunofluorescence showed that ZNF185 was mainly located in the cytoplasmof the sertoli
cells.Conclusion:ZNF185 may affect the process of spermatogenesis by regulating the structure and function of the sertoli cells. |
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