郝广华 汪南平 黄雅姿 赵蓓蕾 张弘.视黄醇结合蛋白4 对血管平滑肌细胞迁移和增殖的影响及机制[J].现代生物医学进展英文版,2016,16(17):3212-3216. |
视黄醇结合蛋白4 对血管平滑肌细胞迁移和增殖的影响及机制 |
Role of RBP4 in Vascular Smooth Muscle Cells Migration and Proliferationand Its Molecular Mechanism |
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DOI: |
中文关键词: 视黄醇结合蛋白 动脉粥样硬化 平滑肌细胞 迁移 增殖 |
英文关键词: Retinol binding protein Atherosclerosis Smooth muscle cell Migration Proliferation |
基金项目:国家自然科学基金项目(30900693);中国博士后科学基金会基金项目(20080440284) |
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中文摘要: |
目的:研究视黄醇结合蛋白4(Retinol-binding protein 4,RBP4)对血管平滑肌细胞(SMCs)迁移和增殖的影响及分子机制。方
法:体外培养大鼠主动脉SMCs,采用划痕实验及Boyden's 迁移小室实验观察RBP4 对SMCs 迁移的影响,采用免疫印迹实验技
术检测Akt 的磷酸化水平,采用Boyden's 小室实验观察PI3K 抑制剂LY294002 预处理细胞对RBP4 促SMCs 迁移的影响,应用
MTT 比色实验结合流式细胞仪技术,检测RBP4 对SMCs 细胞增殖及细胞周期的影响。结果:RBP4 呈剂量依赖性诱导大鼠血管
SMCs迁移(P<0.05);RBP4 处理细胞显著增加了Akt 磷酸化;PI3K 抑制剂LY294002 预处理细胞则显著抑制了RBP4的促迁移
作用(P<0.05);RBP4 处理有增加SMCs数量的趋势,且可轻微阻滞细胞进入S期,但未达到统计学显著性(P>0.05)。结论:RBP4
通过PI3K-Akt通路诱导大鼠血管SMCs迁移,对细胞增殖及细胞周期则无显著影响。 |
英文摘要: |
Objective:To explore a potential role of retinol-binding protein 4 (RBP4) in vascular smooth muscle cells (SMCs) migration
and proliferation and its molecular mechanism.Methods:Vascular SMCs of rat were cultured in vitro. Vascular SMCs migration
was studied by scratch assay and Boyden's chamber. Western blot analysis was used for detecting Akt and phospho-Akt protein levels.
SMCs were pretreated with PI3K inhibitor LY294002, Boyden's chamber was then used for detecting RBP4-induced cell migration. Cell
proliferation and cell cycle of vascular SMCs induced by RBP4 was detected by use of MTT dye absorbance and flow cytometry.Results:Recombinant human RBP4 significantly induced migration of rat vascular SMCs in a dose-dependent fashion (P<0.05) and phosphorylation
of Akt at the same time. PI3K inhibitor Ly294002 significantly suppressed RBP4's effects on cell migration (P<0.05). RBP4 treatment
slightly increased SMCs proliferation and led to G1/S cell cycle arrest, but these effects did not reach statistical significance.Conclusion:RBP4 induces migration of vascular SMCs through PI3K-Akt-dependent pathways. However, the proliferation and cell cycle
progression of the SMCs is not affected by RBP4 treatment. |
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