| 龙兴云 张平 刘艳丰 邓湘 欧立文.氧化苦参碱对哮喘大鼠肺组织IL-10 表达的影响[J].现代生物医学进展英文版,2016,16(16):3036-3039. |
| 氧化苦参碱对哮喘大鼠肺组织IL-10 表达的影响 |
| The Effects of Oxymatrine on the IL-10 Level in Lung Tissues of Asthmatic Rats |
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| DOI： |
| 中文关键词: 支气管哮喘 氧化苦参碱 IL-10 |
| 英文关键词: Asthma Oxymatrine IL-10 |
| 基金项目:湖南省教育厅科研基金项目（12A126） |
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| 中文摘要: |
| 目的：观察不同浓度氧化苦参碱（Oxymatrinem, Oxy）对哮喘大鼠肺组织IL-10 表达的影响，并探讨其作用机制。方法：构建哮
喘大鼠模型，将40 只清洁级健康雌性SD 大鼠随机分成5 组，每组8 只：A：哮喘组（仅卵蛋白（Ovalbumin，OVA）致敏）、B：低浓度
组（Oxy 50 mg/kg）、C：中浓度组（Oxy 100 mg/kg）、D：高浓度组（Oxy 150 mg/kg）、E：对照组（生理盐水），末次激发24 h后处死全部
大鼠，取大鼠肺脏，HE 染色观察肺组织病理改变，采用RT-PCR、Western Blot 测定各组肺组织中IL-10 基因及蛋白水平的表达。
结果：HE 结果显示，哮喘组可见大量炎症细胞浸润，气管平滑肌明显增厚。对照组肺泡壁薄且光滑，未见明显炎性细胞的浸润，不
同浓度氧化苦参碱药物干预组其肺组织炎症细胞浸润及气管平滑肌病变程度随着用药浓度的增高呈逐渐减轻趋势。RT-PCR以
及Western blot检测IL-10 发现，哮喘组、氧化苦参碱低浓度组、氧化苦参碱中浓度组与对照组相比IL-10 的表达均有所减低（P＜
0.05），而氧化苦参碱高浓度组与对照组比较，IL-10 的表达无统计学意义（P＞0.05）；氧化苦参碱中浓度组、氧化苦参碱高浓度组
与哮喘组相比IL-10 的表达均有所增高（P＜0.05），氧化苦参碱低浓度组与哮喘组相比IL-10 的表达无统计学意义（P＞0.05）。结
论：氧化苦参碱抑制、控制哮喘发作可能与促进肺组织中IL-10 基因、蛋白的表达相关，且促进程度在一定范围内与浓度呈正比。 |
| 英文摘要: |
| Objective:To observe the influence of the different concentration of Oxymatrine on the level of IL-10 in lung tissues
of asthmatic rats and explore its mechanism.Methods:40 healthy female SD rats were randomly divided into five groups: A: the asthma
model group(OVA), B: low concentration group(Oxy 50 mg/kg), C: mediumconcentration group(Oxy 100 mg/kg), D: high concentration
group (Oxy 150 mg/kg) and E: Control group (normal saline). The rats were killed when they were nebulizated after 24 hours. The
histological change of lung tissues was observed by HE staining. The expression of IL-10 in lung tissues was measured by RT-PCR and
Western blot.Results:HE stainging demonstrated that a large number of inflammatory cells infiltration existed in lung tissues of
asthmatic group, airway smooth muscle became think, excessive collagen deposition, but it did not obviously change in control group.
inflammatory cells infiltration in lung tissues and airway smooth muscle change in different concentrations of oxymatrine drugs groups
were gradually reduced. The expressions of IL-10 in asthma model group, low concentration group and medium concentration group was
lower than that of control group (P＜0.05), but The expressions of IL-10 in high concentration group had no significant differences
compared with control group (P＞0.05); The expressions of IL-10 in medium concentration group and high concentration group was
higher than asthma model group (P＜0.05), but the expressions of IL-10 in low concentration group had no significant differences
compared with asthma model group (P＞0.05).Conclusion:Oxymatrine can promote the expression of IL-10 in asthmatic rats, which
was positively correlated in the concentration range of Oxy. |
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