| 张浩强 王臻 蓝平衡 李明辉 高鹏
袁玉林 肖鑫 李本强 江新兵 郑毅.量子点应用于人骨肉瘤HOS细胞系研究的生物毒性评价[J].现代生物医学进展英文版,2016,16(16):3005-3009. |
| 量子点应用于人骨肉瘤HOS细胞系研究的生物毒性评价 |
| The Biological Toxicity Study of QuantumDots Applied in the HumanOsteosarcoma HOS Cell Line |
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| DOI： |
| 中文关键词: 量子点 骨肿瘤 骨肉瘤 HOS 细胞系 生物毒性 |
| 英文关键词: Quantumdot Bone tumor Osteosarcoma HOS cell line Biotoxicity |
| 基金项目:国家自然科学基金面上项目（31370944，31170914） |
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| 中文摘要: |
| 目的：将合成的两种量子点应用于研究人骨肉瘤HOS 细胞系，初步检测其生物毒性，以确定本研究所制备量子点可否应用
于骨肉瘤的基础研究。方法：将生长良好的HOS细胞与制备的两种量子点分别共培养，使用MTT 试剂盒检测不同时间点细胞活
性，实验进行三次，取其平均值，分析所得数据，并绘制出量子点浓度- 细胞活性曲线，分析得出两者之间的量效关系。结果：1.4
uM的CdTe/ZnS QDs 和0.275 uM的CdTe/CdS QDs 分别是本实验中对HOS细胞的最高毒性浓度。较短时间(30 min)的暴露分
别导致了48.6± 0.9 %和31.9± 0.8 %的细胞死亡，3 h后分别有33.7± 2.3 %和49.4± 1.1 %的细胞存活。而在孵育了18 h 之后，只
有28.0± 1.6 %和15.3± 1.6 %的细胞存活。我们可以观察到均为典型的时间/ 浓度曲线。结论：选用适宜浓度以及共培养时间，本
实验制备的量子点完全可以满足纳米量子点粒子使用于HOS 细胞研究的基本生物学条件，可以进行人骨肉瘤HOS细胞测温等
的一些列实验操作。由于量子点自身优越的光学性能以及可接受的生物安全性，在肿瘤研究领域具有很大的潜力，将会成为肿瘤
示踪、检测、以及靶向治疗新的有力工具。 |
| 英文摘要: |
| Objective:Two kinds of quantum dots (QDs) was applied to study human osteosarcoma HOS cell line, preliminary
testing its biological toxicity, in order to determine the preparation that the quantum dots we manufacture can be applied in the basic
research of osteosarcoma.Methods:Co-culture the well grow HOS cells with the two kind of QDs respectively, by using the MTT kit to
test thrice the cell viability of different point in time, calculated the average and analyzed the data, then draw the curve of QDs
concentration-cell viability, got the dose-effect relationship.Results:1.4 uM CdTe/ZnS QDs and 0.275 uM CdTe/CdS QDs were the
highest concentration react with HOS cells, respectively. For short time (30 mins) exposure led to 48.6± 0.9 % and 31.9± 0.8 % cells
death, respectively. And after 3 hrs, there were still 33.7± 2.3 %and 49.4± 1.1 %cells alive. And after reacting for 18 hrs, 28.0± 1.6 %
and 15.3 ± 1.6 % cell survival. The curve of QDs concentration-cell viability was a typical time/concentration-dependent curve.Conclusion:By chooseing appropriate concentration and co-culture time, the QDs we made can satisfy the basic biological condition
that applied in HOS cell line, and proper use to the osteosarcoma basic research.Because of the predominant optical performance and
acceptable biosecurity, QDs have a great potential in the tumor research field, and will be powerful instrument for tumor labeling,
detection, and targeted therapy. |
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