| 张欢 李立安 刘芳 张唯一 魏云芝 吴晓洁 郗永义 周艳荣 陈红星 林艳丽.长链非编码RNA Dleu2 对宫颈癌细胞增殖、迁移及侵袭的影响[J].现代生物医学进展英文版,2016,16(15):2801-2805. |
| 长链非编码RNA Dleu2 对宫颈癌细胞增殖、迁移及侵袭的影响 |
| Effect of lncRNA Dleu2 on Proliferation, Migration and Invasion of CervicalCancer Cells |
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| DOI： |
| 中文关键词: 宫颈癌 长链非编码RNA Dleu2 |
| 英文关键词: Cervical cancer LncRNA Dleu2 |
| 基金项目:国家自然科学基金项目（81202445） |
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| 中文摘要: |
| 目的：研究长链非编码RNA（long non-coding RNA，lncRNA）Dleu2 对宫颈癌细胞增殖、迁移和侵袭能力的影响。方法：利用
高通量LncRNA 芯片技术检测10 例宫颈癌组织及对应的癌旁组织，筛选得到一批表达水平具有显著差异的LncRNA，进一步针
对可能具有生物学功能的LncRNA-Dleu2，利用q-PCR 验证其在癌组织样本中的相对低表达。再通过在细胞内过表达LncRNA-
Dleu2 研究其对宫颈癌Hela 和Caski细胞系增殖、迁移和侵袭的影响。结果：q-PCR 结果验证了LncRNA 芯片筛选的结果，即
相较于癌旁组织和正常宫颈上皮细胞系，LncRNA-Dleu2 在宫颈癌组织和细胞中均低表达。CCK8 和克隆形成实验结果显示，过表
达LncRNA-Dleu2能显著抑制Hela 和Caski 细胞增殖能力（P<0.01）；细胞划痕实验结果显示，过表达LncRNA-Dleu2能显著抑制
Hela 和Caski细胞增殖和迁移能力；Matrigel细胞侵袭实验结果显示，过表达LncRNA-Dleu2 能显著抑制Hela 和Caski 细胞的侵
袭能力（P<0.01）。结论：LncRNA-Dleu2 在宫颈癌中相对低表达，提高Dleu2表达水平能够抑制宫颈癌细胞系Hela 和Caski 的增
殖、迁移和侵袭能力。 |
| 英文摘要: |
| Objective:To investigate the potential biological effects of long non-coding RNA Dleu2 on the characteristics of cervical
cancer cells, including items of proliferation, migration and invasion.Methods:High throughput microarray screening of LncRNA
was performed with 10 different cervical cancer tissues as samples and corresponding para-carcinoma tissues as controls, LncRNAs with
significantly different expression levels were selected and among which, the microarray results of LncRNA Dleu2 was further verified by
q-PCR method, then the overexpression of Dleu2 in Hela and Caski, were performed to investigate its potential functions on the proliferation,
migration and invasion of these two cervical cancer cell lines, which characteristic items were quantified separately by CCK8,
clone-forming unit assay, wound healing assay and tumor invasion assay methods.Results:Both the results of Microarray and q-PCR
demonstrated that the expression level of Dleu2 was significantly much lower in cervical cancer tissues and cell lines as compared with
corresponding normal controls. The results of CCK8 assay and colony-forming unit assay showed that Dleu2 overexpression could significantly
decrease cervical cancer cell proliferation. Wound Healing assay results indicated that Dleu2 overexpression could obviously suppress
cervical cancer cell proliferation and migration. Tumour invasion assay results demonstrated that Dleu2 could significantly suppress
cervical cancer cell invasion.Conclusion:The expression levels of Dleu2 are relatively much low in cervical cancer tissues and cell lines.
The overexpression of Dleu2 can significantly inhibit the ability of proliferation,migration and invasion of Hela and Caski cells. |
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