| 李倩 程宣 车冠达 李双双 黄昀 刘轩 邹先伟 陈本科 唐劲天.辣椒碱对3T3-L1 前脂肪细胞葡萄糖摄取的影响[J].现代生物医学进展英文版,2016,16(14):2601-2603. |
| 辣椒碱对3T3-L1 前脂肪细胞葡萄糖摄取的影响 |
| Effects of Capsaicin on Glucose Uptake in 3T3-L1 Preadipocytes |
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| DOI： |
| 中文关键词: 辣椒碱 3T3-L1 细胞 葡萄糖摄取 葡萄糖转运蛋白-1 |
| 英文关键词: Capsaicin 3T3-L1 cells Glucose uptake GLUT-1 |
| 基金项目: |
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| 中文摘要: |
| 目的：探讨辣椒碱对3T3-L1 前脂肪细胞葡萄糖摄取的影响。方法：不同浓度的辣椒碱作用于3T3-L1 前脂肪细胞，采用MTT
测定细胞活性，GLU Test 试剂盒法测定葡萄糖摄取，Western Blot 法检测葡萄糖转运蛋白1（GLUT-1）表达的变化。结果：25 uM
辣椒碱作用72 h和50 uM、100 uM 辣椒碱作用48 h、72 h，可显著抑制3T3-L1 细胞增殖，6.25、12.5、25 uM 辣椒碱作用可显著促
进3T3-L1 细胞的葡萄糖摄入，Western Blot 结果显示辣椒碱能够显著增加GLUT1 蛋白表达量，差异均具有统计学意义（P<0.
05）。结论：低剂量辣椒碱具有降糖作用，其作用机制可能与增加GLUT-1 蛋白表达有关。 |
| 英文摘要: |
| Objective:To evaluate the effect andpotential mechanism of capsaicin on the glucose uptake in 3T3-L1 preadipocytes
in vitro.Methods:3T3-L1 preadipocytes cells were exposed to different concentrations capsaicin for 24 h, 48 h and 72 h. MTT assay was
used to detect their effect on the cell vitality. Glucose metabolism was determined by GLU Test Kits. The GLUT-1 protein was
demonstrated by Western Blot.Results:Treatment of capsaicin (25 uM, 50 uM, 100 uM) showed significant effect on cell viability.
Capsaicin (6.25, 12.5, 25 uM) could improve glucose uptake in 3T3-L1 cells. Western Blot results showed that the glucose transporter 1
(GLUT-1) protein expressions were significantly increased in treatment groups than control.Conclusion:the effect of capsaicin on
glucose metabolismmight be associated with promoting the GLUT-1 protein expression. |
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