| 刘斌 哈尼再尔·热合曼 高阳 李转运 冉新建 罗晶晶 孙湛.黑木耳多糖对内毒素诱导急性肾脏损伤的保护作用及机制研究[J].现代生物医学进展英文版,2016,16(13):2438-2441. |
| 黑木耳多糖对内毒素诱导急性肾脏损伤的保护作用及机制研究 |
| Auricularia Auricular-judae Polysaccharide AttenuatesLipopolysaccharide-induced Acute Kidney Injury by Inhibiting OxidativeStress |
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| DOI： |
| 中文关键词: 黑木耳多糖 脂多糖 急性肾损伤 氧化应激 |
| 英文关键词: Auricularia auricular-judae polysaccharide (AAP) Lipopolysaccharide Acute kidney injury Oxidative stress |
| 基金项目:国家自然科学基金项目（81260454）；新疆维吾尔自治区大学生创新实验项目（CX2014021） |
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| 中文摘要: |
| 目的：探讨黑木耳多糖对内毒素（LPS）诱导大鼠急性肾损害的保护效应及可能机制。方法：健康雄性SD 大鼠，分为正常对
照组、LPS 组和黑木耳多糖组（LPS+ 黑木耳多糖）。根据分组，分别于复制模型前7 d 给予生理盐水或1%黑木耳多糖(15 mL/kg)预
防性灌胃。第8 d 腹腔注射生理盐水或LPS（8 mg/kg），造模12 h 后，通过腹主动脉采血检测血清尿素氮（BUN）、肌酐（Cr）水平，取
肾脏制备匀浆检测肾组织中丙二醛（MDA）、超氧化物歧化酶（SOD）、总抗氧化能力（T-AOC）水平。结果：用黑木耳多糖干预后，大
鼠血清中BUN、Cr 水平均显著低于LPS 组（P<0.05）；LPS 组MDA 含量较正常对照组增高，SOD、T-AOC 水平降低，差异有统计
学意义（P<0.05）；黑木耳多糖组MDA 含量较LPS 组降低，SOD、T-AOC水平升高，差异有统计学意义（P<0.05）。结论：黑木耳多
糖能显著提高内毒素血症大鼠的抗氧化能力，对肾脏组织有保护作用。 |
| 英文摘要: |
| Objective:To investigate the role of Auricularia auricular-judae polysaccharide (AAP) in acute kidney injury induced
by lipopolysaccharide (LPS) in rats and to further explore the possible underlying mechanisms.Methods:Adult Sprague-Dawley rats
were randomly assigned into the control, LPS, and LPS plus AAP groups. Rats were injected with LPS (10 mg/kg, i.p.) to induce acute
kidney injury. Rats in the LPS plus AAP group were treated with AAP for 7 days before the induction of ALI. Blood urea nitrogen(BUN)
and creatinine (Cr) in serum were measured. Superoxide dismutase(SOD), total antioxidant capacity (T-AOC)activity and malondialdehyde
(MDA) level in kidney were measured. Pathological changes of kidney tissues were observed by HE staining.Results:The data
showed that treatment with AAP group significantly improved LPS-induced lung pathological changes, decreased the BUN and Cr level,
and reduced MDA level, increased the SOD, T-AOC activity.Conclusion:The results indicated that AAP has a protective effect on
LPS-induced acute kidney injury in rat. |
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