| 公维东 于咏婧 罗娟 岳子勇△ 白利群.允许性高碳酸血症对大鼠机械通气相关性肺损伤NF-kB 表达的影响[J].现代生物医学进展英文版,2016,16(13):2409-2413. |
| 允许性高碳酸血症对大鼠机械通气相关性肺损伤NF-kB 表达的影响 |
| Effects of Hypercapnia on Expression of NF-kB in Ventilator-InducedLung Injury in Rats |
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| DOI： |
| 中文关键词: 允许性高碳酸血症 机械通气肺损伤 NF-kB |
| 英文关键词: Hypercapnia Ventilator-induce lung injury NF-kB |
| 基金项目:国家自然科学基金项目(30772085) |
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| 中文摘要: |
| 目的：探讨允许性高碳酸血症对大鼠机械通气相关性肺损伤（VILI）时NF-kB 表达的影响。方法：健康雄性Wistar 大鼠30
只，体重220～280 g，采用随机数字表法，将大鼠随机分3 组（n=10)：对照组（C 组）保留自主呼吸、机械通气肺损伤组（V 组）和
VILI+ 高碳酸血症干预治疗组(H 组)行机械通气4 h。采用吸气相高气道压机械通气模式制备机械通气相关性肺损伤模型。H 组通
过调整吸入的CO2浓度来维持动脉血PaCO2分别为80～100 mmHg。于机械通气15 min 时、机械通气1 h、2 h和4 h 时记录
MAP，采集股动脉血样，进行动脉血气分析，记录PaO2；机械通气结束时，测定支气管肺泡灌洗液(BALF)中总蛋白、TNF-琢和巨噬
细胞炎症蛋白-2（MIP-2）的浓度；取肺组织，测定湿干重比（W/D 比）、细胞间粘附分子（ICAM-1）和NF-kb 蛋白的表达水平，并观
察病理学结果，进行肺损伤评分。测定肺组织丙二醛（MDA）含量及过氧化物歧化酶（SOD）活性。结果：与C 组比较，V 组和H 组
肺损伤评分、W/D 比、ICAM-1表达水平、BALF中总蛋白浓度、TNF-alpha和MIP-2 浓度、MDA 含量和肺组织NF-资B 活性升高，PaO2
降低（P<0.05）；与V 组比较，H 组肺损伤评分、W/D 比、ICAM-1表达水平、BALF 中总蛋白浓度、TNF-alpha和MIP-2 浓度和肺组织
NF-kB 表达降低，SOD活性增强，PaO2升高（P<0.05）。结论：允许性高碳酸血症可下调NF-资B的表达，从而抑制炎症反应减轻大
鼠机械通气相关性肺损伤。 |
| 英文摘要: |
| Objective:To investigate the effects of hypercapnia on expression of NF-kB in ventilator lung injury in rats.Methods:thirty male Wistar rats weighting 250～280 g were randomly divided into 3 groups (n=10 each): control group (C group), VILI group(V
group),VILI+ hypercapnia group (H group). A rat model of lung injury induced by ventilation with high peak inspiratory pressure(PIP).
Parameter of ventilation: PIP=25 cm H2O, PEEP=2 cm H2O, the fraction of inspiratory O2 50% and adjusting the level of CO2 to maintained
PaCO2 of group V and group H at 35～45 mmHg and 80～100 mmHg respectively. The rats were sacrificed by exsanguination at
4 h of mechanical ventilation and the lung tissues were removed for microscopic examination, lung wet to dry weight ratio (W/D), determination
of ICMA-1(by immuno histochemistry), NF-kB expression (by Western blotting) and determination of TNF-alpha, MIP-2 (by enzyme
linked immunosorbent assay), MDA and total of protein concentration in bronch-alveolar lavage fluid(BALF).Results:Mechanical
ventilation with high PIP in group V induced serious histopathologic damage, significant increase in TNF-alpha, MIP-2 and total protein concentration
in BALF, ICAM-1 and NF-kB, MDA in lung tissue and W/D ratios as compared with group C（P<0.05）. The indexes above
were significant decreased in group H compared with group V（P<0.05）Conclusion:Hypercapnia attenuated ventilator-induce lung injury
by down regulating the expression of NF-kB that inhibit the expression of inflammatory cytokines in rat lung tissues. |
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