| 侯丽婷 方先海 刘金锋 周华成 李文志.不同频率电针刺激对神经痛模型大鼠脊髓背角P物质的影响[J].现代生物医学进展英文版,2016,16(12):2267-2270. |
| 不同频率电针刺激对神经痛模型大鼠脊髓背角P物质的影响 |
| Effect of Different Frequency of Electroacupuncture on the Expressionof Substance P in the Spinal Cord Dorsal Hornof a Neuropathic Pain Model in Rat |
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| DOI： |
| 中文关键词: P物质 电针 慢性坐骨神经压迫损伤 脊髓背角 |
| 英文关键词: SP Electric acupuncture CCI Spinal dorsal horn |
| 基金项目:黑龙江省卫生厅科研基金项目(2011-093) |
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| 中文摘要: |
| 目的：观察P 物质(Substance P，SP)在慢性坐骨神经压迫损伤(chronic constriction injury，CCI)模型脊髓中表达的变化，探讨电
针镇痛的机制是否与脊髓背角中SP 表达的变化有关。方法：选择32只雄性、体重180-200 g的SD大鼠，并将其随机均分为4组
(n=8)。空白组(Con 组)为正常痛阈值大鼠；假电针组（CCI+A 组）在损伤的坐骨神经旁置入电针，但无电流刺激；2 Hz 组和100Hz
组分别给予相应频率电流刺激30 min。在实验开始前和术后1、4、7、14、20、22 天记录大鼠的热缩足反射潜伏期(Paw Withdrawal
Latency，PWL)和机械刺激缩足反射阈值(Paw Withdrawal Threshold，PWT)。免疫组化方法检测脊髓背角SP的表达。结果：术后20
天，电针治疗后，100 Hz 组和2 Hz组PWT 分别为(7.33± 1.42)g 和(7.80± 1.42)g，均显著高于假电针组(2.60± 1.46)g，差异有统计
学意义(P < 0.05)。100 Hz组在术后20 天后和2 Hz 组在术后14 天后PWL值均显著高于假电针组，差异有统计学意义(P < 0.05)。
免疫组化显示：2 Hz 组和100 Hz 组大鼠脊髓背角中P物质阳性细胞显著低于假电针组(P < 0.05)。结论：坐骨神经旁电针刺激能
够显著减轻CCI模型大鼠热痛觉及机械痛觉过敏，其机制可能与抑制脊髓背角SP的表达有关。 |
| 英文摘要: |
| Objective:To observe the change of Substance P expression in the spinal cord dorsal horn of a chronic constriction
injury (CCI) model in rat, and determine whether the change of Substance P expression in the spinal cord dorsal horn was involved in the
mechanismof electric acupuncture analgesia.Methods:32 adult male SD rats weighed 180-200 g were randomly divided into four groups
(n=8): the control group had normal pain threshold; CCI+A group had injury of sciatic nerve in electric acupuncture without current
stimulation; 2 Hz group and 100 Hz group were given corresponding frequency of electrical stimulation for 30 min. The Paw Withdrawal
Latency (PWL) and Paw Withdrawal Threshold (PWT) were recorded 1, 4, 7, 14, 20, 22 days postoperation and before starting the
experiment. Immunohistochemical method was used to detect the SP expressions in the dorsal horn of the spinal cord.Results:After 20
days, 100 Hz after the electric acupuncture treatment group and 2 Hz PWT respectively (7.33 + 1.42) g and (7.80 + 1.42)g , significantly
higher than that of false electric acupuncture group (2.60 + 1.46) g (P<0.05). 100 Hz after 20 days after surgery, and 2 Hz group 14 days
after surgery PWL value was significantly higher than that of the false electric acupuncture group (P<0.05). Immunohistochemistry
showed that in the 2 Hz and 100 Hz group, substance P in the spinal dorsal horn of the positive cells was significantly lower than the
CCI+A group (P < 0.05).Conclusion:Sciatic nerve by electroacupuncture could significantly reduce the thermal pain and mechanical
hyperalgesia in rat CCI model, the inhibition of SP in the spinal cord dorsal horn might be involved. |
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