| 吕学芬 孙姝 逄明杰 郭菲菲 孙向荣 公衍玲 徐珞.Orexin-A对高脂饮食诱导肥胖大鼠摄食和体重的影响[J].现代生物医学进展英文版,2016,16(12):2236-2240. |
| Orexin-A对高脂饮食诱导肥胖大鼠摄食和体重的影响 |
| Effects of Orexin-A on Food Take and BodyWeightof Diet-induced Obesity Rats |
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| DOI： |
| 中文关键词: Orexin 下丘脑 饮食诱导肥胖 高脂饮食 SPA |
| 英文关键词: Orexin Hypothalamus Diet-induced obesity High-fat diet Spontaneous physical activity |
| 基金项目:国家自然科学基金项目(81470815，31071014，81100260，81270460，81300281，81500414)；
山东省优秀中青年科学家科研奖励基金项目（BS2014YY009）；青岛市科技局项目（13-1-4-170-jch, 14-2-3-3-nsh） |
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| 中文摘要: |
| 摘要目的：探讨Orexin-A对高脂饮食诱导的肥胖大鼠摄食和体重的影响。方法：通过检测SD 大鼠24 h动态SPA的分布情况来
定义HA 大鼠和LA 大鼠，创建饮食诱导肥胖（DIO）大鼠模型，向HA 和LA 大鼠延髓外侧下丘脑（rLH）和黑质多巴胺致密部
（SN））微量注射orexin-A，观察orexin-A 对能量消耗、自发动态运动（SPA）的作用，以及动态SPA 对饮食诱导肥胖（DIO）的抵抗
作用。结果：雄性SD 大鼠在标准饮食情况下，24 h动态SPA 呈正偏态分布，非固定SPA（ambulatory SPA）与瘦体重(lean mass,
LM)（P < 0.05)以及总体重(P < 0.05)显著相关。HA和LA 大鼠（high and low activity rats）间的固有SPA（intrinsic SPA）差异有显著
统计学意义(P < 0.05)。与LA 大鼠相比，HA 大鼠延髓外侧下丘脑（rLH）和黑质多巴胺致密部（SN）的orexin-A 反应性更高。在rLH
和SN 注射orexin-A能显著增加动态SPA (rLH: P < 0.05; SN: P < 0.05)。在HA和LA 大鼠的rLH 注射orexin-A，每种剂量与注射
相同剂量的aCSF的大鼠相比效果有显著差异。而对于SN 注射OXA，只有注射高剂量OXA 的HA大鼠，与对照组相比，才出现
著差异。在rLH 注射OXA后，对HA/LA 大鼠动态SPA 均有显著影响(P < 0.05)。HA大鼠比LA 大鼠能量消耗更高。不同的饮食
对于HA和LA 大鼠转化为SPA 有不同的影响，HA 大鼠对DIO 敏感性低于LA 大鼠。与LA 大鼠相比，在LF(Low Fat)饮食条件
下，转化为脂肪量的热量更少。结论：Orexin-A 可通过增加大鼠活动量，使高脂饮食诱导的肥胖大鼠体重减轻。 |
| 英文摘要: |
| Objective:To investigate the effect of orexin-A on food take and body weight in diet-induced obesity rats.Methods:After acclimation to the housing facilities, male Sprague-Dawley (SD) rats were classified rats as HA or LA on the basis of measurement
of ambulatory and stereotypic SPAINT. Some of HA and LA rats were used to establish DIO models. Rats were prepared with unilateral
cannulae targeting rLH or SN and orexin-A was injected as schedule. Body weight, fat and lean mass, food intake, SPA, indirect
Calorimetry (IDC) of rats was measured.Results:There were significant differences in SPA between HA and LA rats but similar total
body weight, fat mass. Higher level of ambulatory SPA was correlated with higher rLH orexin responsivity, and orexin regulation of SPA
was distributed through multiple brain sites including the rLH and SN. Therefore, we proposed that HA rats might have higher rLH and
SN orexin responsivity compared to LA rats. There were significant differences in rLH of HA and LA rats across all doses of orexin
among individual doses of OXA against aCSF injection. On the contrary, for SN, there were only significant differences within HA rats at
higher OXA. After adjustment for total body mass, HA rats showed significantly higher EE than LA rats (P＜0.05) while there were no
differences in uncorrected EE between HA and LA rats averaged over IDC recording sessions doses. LA rats had higher DIO sensitivity
than HA rats.Conclusion:OrexinA signaling contributes to the observed variations in SPA and DIO sensitivity. |
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