| 祝加学 陆雄伟 胡小鹏 顾剑华 王健.磁纳米颗粒标记脂肪干细胞向软骨分化及体外MRI示踪的实验研究[J].现代生物医学进展英文版,2016,16(12):2209-2212. |
| 磁纳米颗粒标记脂肪干细胞向软骨分化及体外MRI示踪的实验研究 |
| In Vitro MRI Monitoring Magnetic Iron Oxide Particles(MIOP) LabelingAdipose Derived Stem Cells Differentiation into Chondrocytes |
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| DOI： |
| 中文关键词: 软骨分化 脂肪间充质干细胞（ASCs） 磁纳米铁颗粒（MIOP） MRI示踪 |
| 英文关键词: Chondrocytes differentiation Adipose derived stem cells (ASCs) Magnetic iron oxide particles (MIOP) Magnetic
monitoring |
| 基金项目:上海交通大学医学院基金项目(13XJ10055)；上海市卫生局科研基金项目(20134195) |
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| 中文摘要: |
| 目的：探讨磁纳米颗粒（magnetic iron oxide particles, MIOP）体外标记脂肪间充质干细胞（ASCs）向软骨分化及MRI 示踪的
可行性。方法：从小鼠脂肪组织中分离培养、扩增脂肪间充质干细胞（ASCs），流式鉴定细胞表型后，分别采用不同浓度（25 ug/mL，
50 ug/mL）的MIOP标记ASCs 并向软骨细胞诱导分化。普鲁士蓝染色和透射电镜（TEM）鉴定细胞内磁纳米铁颗粒分布情况，应
用3.0T MRI体外检测标记软骨细胞MRI信号。结果：从脂肪组织中可以分离获得大量高表达CD90、CD105、Sca-1 的ASCs, 不同
浓度（25 ug/mL，50 ug/mL）的MIOP与ASCs 共同孵育24 小时后，普鲁士蓝染色发现ASCs随MIOP浓度的增加，蓝染程度逐渐
加深且标记的ASCs可以向软骨细胞分化；TEM 证实细胞内分布大量的黑色纳米铁颗粒。体外MRI T2 序列证实随着MIOP 浓度
（25 ug/mL，50 ug/mL）的增加MRI信号值逐渐减低且具有统计学差异（P< 0.05）。结论：MIOP 可以标记ASCs 向软骨分化，体外
应用MRI 可以对其进行示踪 |
| 英文摘要: |
| Objective:To explore the feasibility and effects of labeling Adipose derived stem cells (ASCs) differentiation into
chondrocytes with magnetic iron oxide particles (MIOP), and to investigate the possibility of in vitro MR monitoring.Methods:Adipose
derived stem cells (ASCs) were isolated, expanded from mouse fat tissues, P3 ASCs were tested by Flow cytometry and labeled with
different concentration of MIOP (25 ug/mL, 50 ug/mL) and differentiated into chondrocytes. Prussian blue staining and Transmission
Electron Microscope (TEM) were performed to show the intracellular iron particles. In vitro 3.0 MRI were used to test the signal
intensity of labeled cells.Results:ASCs were successfully cultured and high expression CD90, CD105, Sca-1. After ASCs were cultured
with different concentration of MIOP (25 ug/mL, 50 ug/mL) about 24 hours. With the MIOP concentration increase from 25 ug/mL to
50 ug/mL, the dyeing degree of labeling cells stained by Prussion blue staining deepened and the labeled ASCs could differentiate into
chondrocytes. TEMindicated that a lot of black iron particles accumulated in the cells. In vitro 3.0 MRI results show that T2 intensity of
labeling cells were inversely correlated with the MIOP (25 ug/mL, 50 ug/mL)（P< 0.05）compared with the control group.Conclusion:Adipose derived stemcells could be labeled effectively with MIOP and differentiate into chondrocytes, and in vitro MRI could be used to
monitor these labeled cells. |
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