| 王薇 孙素真△ 陈芳 石仲仁 张梅杰.生酮饮食对难治性癫痫患儿滤泡辅助性免疫细胞的影响[J].现代生物医学进展英文版,2016,16(11):2106-2108. |
| 生酮饮食对难治性癫痫患儿滤泡辅助性免疫细胞的影响 |
| Influence of Ketogenic Diet on the Follicular Helper T Cells and B Cells ofIntractable Epilepsy Patients |
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| DOI： |
| 中文关键词: 难治性癫痫患儿 生酮饮食 滤泡辅助性T 细胞 mTOR |
| 英文关键词: Intractable epilepsy Ketogenic diet Follicular helper T cells mTOR |
| 基金项目:河北省卫生厅科研项目（20130080） |
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| 中文摘要: |
| 目的：探讨难治性癫痫患儿应用生酮饮食对其滤泡辅助性T 细胞及B 细胞的影响。方法：将32 例难治性癫痫患儿设为实验
组，选取正常同龄对照20 例，流式细胞术检查生酮饮食治疗前后Tfh 细胞及B 细胞比例，ELISA 检测IL-21 血清中浓度，RT-PCR
检测Blimp-1、Bcl-6、mTOR 基因表达情况。结果：生酮治疗后Tfh 细胞数量明显下降，Tfh 细胞抑制因子Blimp-1 表达降低，转录
相关因子Bcl-6 表达也降低，血清中IL-21 浓度及mRNA表达均显著减低，CD27+IgD-B细胞、CD27+IgD-BCD38bigb 与治疗后相
比显著下降。治疗后Tfh 细胞及各阶段B 细胞与mTOR 表达存在正相关关系。结论：KD 治疗通过mTOR 降低了Tfh 细胞与B细
胞比例或是抑制了Tfh 细胞与B细胞的功能，这可能是导致接受KD 治疗过程中少数患儿出现低丙种球蛋白血症原因。 |
| 英文摘要: |
| Objective:To explore the influence of ketogenic diet on the follicular helper T cells and B cells of intractable epilepsy
patients.Methods:The 32 patients of intractable epilepsy were selected and 20 children at the same age as control. Before and after the
ketogenic diet, the proportion of Tfh cells and B cells was detected by flow cytometry. IL-21 concentration in serum was detected by
ELISA. And the gene expression of Blimp-1, Bcl-6 and mTOR were detected by RT-PCR.Results:After Ketogenic treatment, Tfh cells
significantly decreased, and the expression of Blimp-1 and Bcl-6, serum concentration and mRNA expression of IL-21 were significantly
reduced. The CD27+ IgD-B and CD27+ IgD-BCD38bigb cells declined significantly after treatment. Tfh cells after treatment and the B
cells in each phase were positively correlated with mTOR expression.Conclusion:KD treatment reduced the proportion of Tfh cells and
B cells or inhibited the function of Tfh cells and B cell function by mTOR. And it may be lead to lowgamma globulin hematic disease after
KD treatment in a minority of children with intractable epilepsy. |
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