| 邵伟怡 苏文利 朱文献 王艳华 王毅鑫 宋振顺.PCT及CRP在急性肠梗阻大鼠血清中的表达水平及其临床意义[J].现代生物医学进展英文版,2016,16(10):1854-1857. |
| PCT及CRP在急性肠梗阻大鼠血清中的表达水平及其临床意义 |
| Expression of PCT and CRP in the Plasma and SerumTissues of Rats withAcute Intestinal Obstruction and Its Clinical Significance |
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| DOI： |
| 中文关键词: 急性肠梗阻 降钙素原 C- 反应蛋白 |
| 英文关键词: Acute intestinal obstruction PCT CRP |
| 基金项目:上海市教委科研基金项目（2012JW72） |
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| 中文摘要: |
| 目的：探讨降钙素原（Procalcitonin，PCT）及C 反应蛋白（C-reactive protein，CRP）在急性肠梗阻大鼠血清中的水平及其临床
意义。方法：将83 只Wistar大鼠分为对照组（n=13）、假手术组（n=35）和急性肠梗阻组（n=35）。对照组大鼠采集标本后处死，肠梗
阻组行开腹手术结扎回肠末端，假手术组仅行开腹手术。检测8 h、24 h、48 h、72 h及96 h血清PCT 及CRP 水平，观察急性肠梗阻
大鼠回肠组织的病理学改变情况。结果：假手术组PCT 与CRP 水平在术后24 h内显著升高，48 h至96 h逐渐下降；各时间点
PCT 水平明显高于对照组，而CRP 水平在实验结束时已恢复至正常水平。肠梗阻组PCT 和CRP 水平在各时间点均明显高于对
照组，并逐渐增加，到实验结束时达到高峰；肠梗阻组PCT 和CRP水平在48 h-96 h均显著高于假手术组，差异具有统计学意义
（P<0.05）。组织病理学检查显示，对照组大鼠肠壁粘膜结构正常，假手术组可见轻度病理改变，肠梗阻组大鼠回肠组织可见粘膜结
构明显破坏，绒毛坏死，严重水肿和炎症细胞浸润。结论：血浆PCT 和CRP 水平能够反映肠梗阻的状态和肠粘膜受损程度。 |
| 英文摘要: |
| Objective:To investigate the level and clinical significance of procalcitonin (PCT) and C-reactive protein (CRP) in
plasma of rats with acute intestinal obstruction.Methods:83 Wistar rats were randomly divided into control group (n=13), sham
operation group (n=35) and acute intestinal obstruction group (n=35). The rats in control group were sacrificed, the rats in the shamgroup
were only taken the open surgery, and the rats in the intestinal obstruction group were taken the open surgery and the ligation of the
terminal ileum. The serum levels of CRP and PCT were detected at different time points, and the pathological changes of ileum in rats
with acute intestinal obstruction were observed.Results:The level of PCT and CRP in sham operation group was gradually increased at 8
h and 24 h and decreased gradually at 48 h, 72 h and 96 h, and the level of PCT was higher than that of control group at different time
points, but the level of CRP at 96 h was returned to control. The level of PCT and CRP in intestinal obstruction group at different time
points were significantly higher than those of the control group, and it was gradually increased to the peak at the end of the experiment,
and the level of PCT and CRP in intestinal obstruction group at 48 h, 72 h and 96 h were significantly higher than those in shamoperation
group with statistical significance (P<0.05). Histopathological examination showed that the structure of the intestinal wall in the control
group was normal, and mild pathological changes were detected in the sham operation group, and the ileum tissues of the intestinal
obstruction group were obviously damaged, with the villi necrosis, severe edema and inflammatory cell infiltration.Conclusion:The
levels of PCT and CRP can reflect the state of intestinal obstruction and the extent of intestinal mucosal damage. |
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