| 王晓琳 翁孝刚 王宪华 王涛 尹清风.黄芪多糖预防2 型糖尿病大鼠肝损伤的实验研究[J].现代生物医学进展英文版,2016,16(10):1846-1849. |
| 黄芪多糖预防2 型糖尿病大鼠肝损伤的实验研究 |
| Experimental Study of Astragalus Polysaccharides on the Liver Injuryin Type 2 Mice |
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| DOI： |
| 中文关键词: 黄芪多糖 2 型糖尿病 肝损伤 氧自由基 |
| 英文关键词: Astragalus Polysaccharides Type 2 diabetes Liver injury Oxygen Species |
| 基金项目:河南省研究生科研创新支持计划课题(HY14M032) |
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| 中文摘要: |
| 目的：研究分析黄芪多糖(Astragalus polysaecharides, APS)在预防2 型糖尿病大鼠肝损伤的作用。方法：Wistar大鼠72 只，其
中48 只以小剂量链脲佐菌素(Streptozotocin, STZ)建立2 型糖尿病大鼠模型，并随机分为模型对照组(B 组)和APS 组(C 组)，余24
只大鼠为正常对照组(A 组)。C 组大鼠给予APS 400 mg/kg/d 灌胃，A、B 组分别给予等量生理盐水。分别于第0、2、4、6 周检测3组
大鼠血清谷丙转氨酶(ALT)、谷草转氨酶(AST)、超氧化物歧化酶(SOD)以及丙二醛(MDA)水平，透射电镜下观察3 组大鼠肝细胞
超微结构变化。结果：第0、2 周时B、C 组大鼠ALT、AST、SOD以及MDA 水平比较无显著差异(P>0.05)。第4、6 周时，C组大鼠
ALT、AST 以及MDA 水平均高于A组，但低于B 组；而SOD 水平低于A组，但高于B 组；差异均有统计学意义(P<0.05)。电镜下
观察，A 组肝细胞内大量线粒体、内质网，结构完好，未见损伤；B组细胞器数量减少，线粒体及内质网形态异常，膜结构破损，细胞
空化；C 组细胞器也存在损伤，但较B 组明显减轻。结论：APS 可增加肝细胞抗氧化酶的活性，促进氧自由基的清除，减少肝细胞
损伤，对预防2 型糖尿病引起的肝功能损害有一定的价值。 |
| 英文摘要: |
| Objective:To investigate the effect of astragalus polysaccharides (APS) on liver injury in diabetic rats.Methods:The
rats model with type 2 diabetes (n=48) were induce by small dose injection of STZ and high calorie feeding, and randomly divided into
diabetic model group(group B) and APS group(400 mg/kg/d) (group C). The 24 rats without treatment were normal control(group A).
The level of AST, ALT, SOD and MDA were detected and analyzed in 0, 2, 4, 6 weeks respectively. And the ultrastructural changes of
the hepatic tissues were observed by transmission electron microscopy.Results:There was no significant difference in AST, ALT, SOD
and MDA between B and C groups in 0, 2 weeks (P>0.05). The AST, ALT and MDA in group C were significantly higher than group A
in 4, 6 weeks (P<0.05), but significantly lower than group B (P<0.05). The SOD in group C were significantly lower than group A in 4, 6
weeks (P<0.05), but significantly higher than group B(P<0.05). The results observed by electron microscopy revealed a large number of
chondriosome and rough endoplasmic reticulum without damage in group A at the 6 weeks, the number of chondriosome and
glycogenosome was decreased, cell cavitation occurred, and the membrane structure and rough endoplasmic reticulum was broken in
group B while the degree of damage of both chondriosome and rough endoplasmic reticulum was obviously milder in group C than in
group B.Conclusion:APS can increase the activity of antioxidant enzymes in hepatocytes, promote the removal of oxygen species and it
can ameliorate the liver injury of type 2 diabetic rats. |
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