| 郭俊 刘宇 王忠凯 李国然 沈下贤 赵仙先.氧化应激诱导内皮细胞凋亡microRNA表达改变的研究[J].现代生物医学进展英文版,2016,16(10):1821-1824. |
| 氧化应激诱导内皮细胞凋亡microRNA表达改变的研究 |
| Study of microRNA Change in Oxidative Stress Induced Endothelial Cells Apoptosis |
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| DOI： |
| 中文关键词: 微小RNA 内皮细胞 凋亡 氧化应激 |
| 英文关键词: microRNA Endothelial cells Apoptosis Oxidative stress |
| 基金项目:国家自然科学基金面上项目（81370266） |
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| 中文摘要: |
| 目的：研究氧化应激诱导的内皮细胞microRNA 的表达变化。方法：ECM（Endothelial Cell Medium）培养人脐静脉内皮细胞，
利用不同浓度双氧水（0 umol/L，200 umol/L，500 umol/L，800 umol/L）刺激24 小时后应用流式细胞术检测其凋亡水平。提取细胞
总RNA，利用实时定量PCR(Quantitive real-time PCR；qRT-PCR)检测microRNA表达量变化，并利用生物信息学软件预测可能的
靶基因。结果：加入不同浓度双氧水处理24 h后的内皮细胞总凋亡率均显著高于对照组，200 umol/L、500 umol/L和800 umol/L
组的凋亡率分别为（13.31%vs 4.75%，35.9%vs 4.75 %，89.75%vs 4.75%，P<0.01）。200 umol/L的双氧水处理内皮细胞后，microRNA
的表达出现了明显的改变。其中miR-92a、miR-126 的表达明显下调（P<0.05），miR-181a、miR-217、miR-34a 和miR-320 的表达
明显上调（P<0.05）。靶基因预测显示miR-320、miR-92a 可能调控多个和内皮细胞凋亡相关的基因表达。结论：在氧化应激诱导的
内皮细胞凋亡中，miRNA 表达发生改变并可能参与调控内皮细胞功能。 |
| 英文摘要: |
| Objective:To determine the expression change of microRNA in endothelial cells under oxidative stress.Methods:Human Umbilical Vein Endothelial Cells cultured by ECM, were stimulated by different concentrations of hydrogen peroxide (0 umol/L,
200 umol/L, 500 umol/L, 800 umol/L) for 24 hours. Cells apoptosis assessed by Annexin V/PI staining and flow cytometry. Total RNA
was isolated from cells, and microRNA expression levels were detected by using quantitive real-time PCR. Bioinformatics prediction
software were applied to predict potential target genes of these miRNAs.Results:The apoptosis rate of endothelial cells increased
significantly after hydrogen peroxide stimulation. Compared with control cells, the apoptosis rate of 200 umol/L, 500 umol/L and 800
umol/L hydrogen peroxide treatment group were significantly increased (13.31 % vs 4.75 %, 35.9 % vs 4.75 %, 89.75 % vs 4.75 %,
respectively, P<0.01). Quantitive real-time PCR results showed that the expression of miRNA appeared obviously disorder, of which
miR-92a, miR-126 were decreased, while miR-181a, miR-217, miR-34a and miR-320 were increased notably (P<0.05). MiR-92a and
miR-320 might regulate the expression of multiple genes related to the apoptosis of endothelial cells.Conclusion:The abnormal
expression miRNAs in HUVECs after oxidative stress induced apoptosis suggested that these miRNAs maybe involved in regulation of
endothelial cells function. |
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