| 杨靖 柴家科 刘玲英 尹会男 胡泉.血管紧张素-II（Ang-II）处理后的人脐带MSCs上清液对HUVEC 凋亡增殖的影响[J].现代生物医学进展英文版,2016,16(10):1816-1820. |
| 血管紧张素-II（Ang-II）处理后的人脐带MSCs上清液对HUVEC 凋亡增殖的影响 |
| Effects of Conditioned MediumDerived fromMesenchymal StemCellsPretreated with Angiotensin-II on Apoptosis and Proliferation of HumanUmbilical Vein Endothelia Cells |
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| DOI： |
| 中文关键词: HUVEC 人脐带MSCs 血管紧张素-II 增殖 凋亡 |
| 英文关键词: Human umbilical vein endothelia cells Human umbilical cord mesenchymal stemcells Ang-II Proliferation Apoptosis |
| 基金项目:国家自然科学基金项目（81372052） |
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| 中文摘要: |
| 目的：体外观察100 ng/mL 血管紧张素-II（Angiotensin-II，Ang-II）处理后的人脐带间充质干细胞（human umbilical cord
MSCs，hUCMSCs）上清液对损伤的人脐静脉内皮细胞（human umbilical vein endothelia cells，HUVEC）增殖、凋亡的影响。方法：
CCK8 法检测HUVEC 增殖情况；倒置显微镜下观察HUVEC 形态；吖啶橙/ 溴化乙锭染色法(acridineorange/ethidium bromide，
AO-EB)、Hoechst 检测HUVEC 凋亡情况。结果：CCK8 实验结果显示，加入100 ng/mL Ang-II 处理后的hUCMSCs 上清液组
HUVEC增殖速度在各时间点显著快于其他两组。倒置显微镜下观察细胞的形态：加入100 ng/mL Ang-II 处理后的hUCMSCs 上
清液组HUVEC 的形态最佳且凋亡数最少。用各组细胞上清液对HUVEC 培养24 h、48 h、72 h后，AO-EB、Hoechst 染色结果显
示：加入100 ng/mL Ang-II处理后的hUCMSCs上清液组，在各个时间点HUVEC出现凋亡细胞或死亡细胞的数最少。结论：100
ng/mL Ang-II预处理后的hUCMSCs 上清液可以促进HUVEC 增殖、抑制HUVEC 凋亡。 |
| 英文摘要: |
| Objective:To investigate the effects of conditioned medium derived from mesenchymal sterm cells (hUCMSCs)
pretreated with 100 ng/mL Ang-II on the proliferation and apoptosis of human umbilical vein endothelia cells (HUVEC) in vitro.Methods:The cell proliferation curve was measured by CCK8, The cell morphology and density were observed by inverted microscope,
and the apoptosis was assessed by AO-EB staining and Hoechst staining.Results:The results of CCK8 showed that the Cells value-addup
of 100 ng/mL Ang-II pretreatment was significantly faster than the other two groups at every time point; the morphology of the cells was
observed under an inverted microscope, The results showed the morphology of HUVEC in the supernatant of 100 ng/mL was the best and
the number of apoptosis was the least. The HUVEC were cultured in the conditioned medium of each group after 24 h, 48 h and 72 h,
The results of AO-EB and Hoechst staining shows the number of apoptotic cells or dead cells in group of 100 ng/mL Ang-II pretreatment
was the least at each time point.Conclusion:The conditioned medium of hUCMSCs which pretreated with 100 ng/mL Ang-II promoted
HUVEC proliferation and inhibited HUVEC apoptosis. |
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