| 白生宾 才仁 李甜 廖礼彬 秦纹 冯树梅 张亚楼 李艳 陈龙 钟近洁△.基于体视显微镜观察两种鼠氟斑牙的形态变化[J].现代生物医学进展英文版,2016,16(8):1458-1461. |
| 基于体视显微镜观察两种鼠氟斑牙的形态变化 |
| Morphology Changes of Dental Fluorosis in Rats/Mice under Digital StereoMicroscope |
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| DOI： |
| 中文关键词: 体视显微镜 Wistar 大鼠 C57BL/6J 小鼠 氟斑牙 |
| 英文关键词: Stereo microscope Wistar rat C57BL/6J mouse Dental fluorosis |
| 基金项目:新疆维吾尔自治区自然基金项目（2013211B54） |
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| 中文摘要: |
| 目的：通过对染氟大鼠和小鼠氟斑牙的观察，进一步确定氟斑牙作为慢性氟中毒的诊断指标的重要性。方法：通过不同浓度
和不同时间的氟染毒，利用数码体视显微镜观察Wistar大鼠和C57BL/6J 小鼠氟斑牙的形态变化。将Wistar 大鼠随机分为4组，
每组8 只，共计32 只，分笼饲养，C57BL/6J 小鼠4 组，每组12 只，共计48 只，分8 笼饲养。对照组：蒸馏水组，实验组三组：氟化钠
分别为50 mg/L组，100 mg/L组和150 mg/L组。在染氟至6 个月时，应用在体视显微镜观察大鼠和小鼠牙齿的动态改变。结果：染
氟组Wistar大鼠和C57BL/6J 小鼠牙齿均出现规则的斑纹、白垩状、延迟断裂、缺损等症状。随着染氟时间的延长，C57BL/6J 小鼠
氟斑牙的损伤程度大于Wistar大鼠。在慢性氟中毒大鼠和小鼠的牙齿变化中发现，大鼠和小鼠的牙齿变化程度与种属有着密切
的关系。结论：通过利用体视显微镜对Wistar大鼠和C57BL/6J 小鼠染氟6 个月进行氟斑牙的观察分析，为慢性氟中毒鼠模型提
供氟斑牙的形态学的详实依据。并且，可根据研究需要适宜选择氟斑牙的动物模型。 |
| 英文摘要: |
| Objective:To observe the morphological changes of dental fluorosis of Wistar rats and C57BL/6J mice by using digital
stereo microscope and to further investigate the value of dental fluorosis as a diagnostic indicator of fluorosis.Methods:A total of 32
Wistar rats were randomly divided into 4 groups (8 rats in each group, feeding in the cage). 48 C57BL/6J mice were randomly divided
into 4 groups (12 mice in each group, feeding in 8 cages). Control group was given sodium, while three experimental groups were
respectively treated with 50 mg/L,100 mg/L and 150 mg/L of sodium fluoride. The dynamic cha-nges of the teeth of rats and mice were
observed under the stereo microscope in the dyeing to 6 months.Results:The Wistar rats and C57BL/6J mice had a series of symptons:
regular pattern of speckle, chalk, delayed fracture and defect. With the extension of time of fluorine dyeing, the damage degree of dental
fluorosis in C57BL/6J mice was more serious than that in Wistar rats. The dental changes of chronic fluorosis rats and mice showed that
there was a close relationship between the degree of tooth change and the species of the rats and mice.Conclusion:This study can
provide detailed data of the morphology of dental fluorosis for rats/mice models of chronic fluorosis,and an animal model of dental
fluorosis can be appropriately chosen according to the research needs. |
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