| 郭伦华 尹飞飞 杨春 陈晓宁 孙世波.CXCL1 在原发性肝癌中的表达及对人肝癌HepG2细胞增殖的影响[J].现代生物医学进展英文版,2016,16(8):1432-1434. |
| CXCL1 在原发性肝癌中的表达及对人肝癌HepG2细胞增殖的影响 |
| The Expression of CXCL1 in Hepatocellular Carcinoma Tissue and Its Rolein the Proliferation of HepG2 Cells |
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| DOI： |
| 中文关键词: CXCL1 人肝癌HepG2 细胞 CCK8 试剂盒 细胞增殖 |
| 英文关键词: CXCL1 HepG2 cells Cell Counting Kit(CCK8) Cell proliferation |
| 基金项目: |
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| 中文摘要: |
| 目的：探究CXCL1 在原发性肝癌中的表达及对人肝癌HepG2细胞增殖的影响。方法：应用免疫组织化学技术（SP 二步法）
检测48 例原发性肝癌组织、13正常肝脏组织中CXCL1 表达情况；通过CCK8 试剂盒检测CXCL1 对HepG2 细胞增殖能力的影
响。结果：CXCL1 在77.1%的原发性肝癌组织中表达，同时CXCL1 在原发性肝癌组织中的表达高于正常肝脏组织，差异有统计学
意义(P<0.01)；不同浓度CXCL1 处理人肝癌HepG2细胞后，人肝癌HepG2细胞增殖能力明显增强，并且在一定范围内存在明显
的剂量效应。结论：CXCL1 在原发性肝癌中表达上调；CXCL1 能够促进人肝癌HepG2 细胞增殖。 |
| 英文摘要: |
| Objective:To study the expression of CXCL1 in hepatocellular carcinoma tissue and its role in the proliferation ofHepG2
cells.Methods:Detect the expression of CXCL1 in 48 cases of HCC tissues and 13 normal liver tissues with Immunohistochemistry
technique (SP method); Detect the effect of CXCL1 on proliferation in HepG2 cells with Cell Counting Kit(CCK8).Results:In 48 cases
of HCC tissues, the expression rate of CXCL1 is 77.1%, and the expression of CXCL1 in HCC tissues is significantly higher than that in
normal human liver tissues, the results was statistically significant (P<0.01).Conclusion:The expression of CXCL1 was up-regulated in
HCC; the constitutive expression of CXCL1 promotes the proliferation of HepG2 cells. |
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