| 杨勇琴 左绍远 张海洋 张晓娟 余敏 熊伟.人线粒体转录终止因子2 蛋白的生物信息学分析[J].现代生物医学进展英文版,2016,16(5):944-949. |
| 人线粒体转录终止因子2 蛋白的生物信息学分析 |
| Bioinformatic Analysis of Human Mitochondrial Transcription TerminationFactor 2 (MTERF2) |
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| DOI： |
| 中文关键词: 人类 线粒体转录终止因子2 生物信息学 蛋白功能 |
| 英文关键词: Homo Sapiens Mitochondrial transcription termination factor 2 Bioinformatics Protein function |
| 基金项目:国家自然科学基金项目（81560458；31260276）；云南省教育厅科学研究重点项目（2014Z126）；
云南省昆虫生物医药研发重点实验室开放课题（201409）；大理学院博士科研启动基金项目（BSKY2012018）；
云南省大学生创新创业计划项目（201408） |
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| 中文摘要: |
| 目的：探讨人线粒体转录终止因子2（mitochondrial transcription termination factor 2, MTERF2）的生物学功能。方法：采用生
物信息学的方法和工具对人MTERF2 的理化性质、跨膜区和信号肽、二级结构功能域、亚细胞定位、蛋白质的功能分类预测、多重
序列比对与系统发育树构建、三级结构建模进行系统的分析与研究。结果：人MTERF2 蛋白分子量为44.41 kD，等电点为9.15，不
具有信号肽和跨膜区。该蛋白定位于细胞线粒体中，N 端1-35 个氨基酸为前导肽序列，其二级结构主要构成元件为螺旋和无规
则卷曲，包含4 个MTERF基序。三级结构预测显示该蛋白为球状结构，N端为螺旋结构，C 端为无规则卷曲。对该蛋白进行多重
序列比对和聚类分析显示，人MTERF2 属于直系同源蛋白质，与猩猩MTERF2 相似性最高，二者在系统发育树上聚为一类。结
论：人MTERF2 是一种典型的线粒体DNA结合蛋白质，本研究为进一步探索人MTERF2 蛋白的功能提供参考资料和理论依据。 |
| 英文摘要: |
| Objective:To investigate biological function of the human mitochondrial transcription termination factor 2 (MTERF2).Methods:Many bioinformatic methods were used to analyze its physical and chemical properties, hydrophobicity, transmembrane region,
signal peptide, secondary structure, functional domain and assortment, multiple alignment, phylogenetic tree and three-dimensional structures.Results:Human MTERF2 belongs to the hydrophilic protein. The calculated molecular mass is 44.41 kD. The theoretical isoelectric
point is 9.15. The signal peptide and transmembrane regions can not be found in human MTERF2. It is localized in mitochondria.
And its N-terminal 1-35 amino acids are transit peptide. The main compositions of protein secondary structure are-helix and random coil,
which contains four MTERF motifs. The prediction of the three-dimensional structure shows a globular structure which have-helix in the
N end and random coil in the C end. The results of the multiple alignment and clustering analysis show that human MTERF2 is a kind of
orthologous protein belonging to MTERF family. It is the most similar to Pongopygmaeus MTERF2. Both the proteins cluster together.Conclusion:Human MTERF2 is a kind of typical mitochondrial DNA binding protein. Our research can provide some useful information
for the further study of the function of human MTERF2. |
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