| 石一彤 季海波 张云玲 林娜 王卓然.食源性肥胖对SD大鼠卵泡发育相关激素及瘦素水平的影响[J].现代生物医学进展英文版,2016,16(5):825-827. |
| 食源性肥胖对SD大鼠卵泡发育相关激素及瘦素水平的影响 |
| The Influence of Diet-induced Obesity on the Level of SerumSex Hormoneand Leptin in SD Rats |
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| DOI： |
| 中文关键词: 食源性肥胖 瘦素 血脂 SD 大鼠 卵泡发育 |
| 英文关键词: Diet-induced obesity Leptin Blood lipid SD rats Follicular development |
| 基金项目:黑龙江省教育厅科研项目（12521287） |
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| 中文摘要: |
| 目的：通过建立大鼠肥胖模型，检测其血脂水平与一系列卵泡发育相关激素指标，试探讨肥胖对大鼠卵泡发育相关激素的
影响及可能机制。方法：从30 只6-7 周龄的SD雌性大鼠中随机选取10 只采用普通饲料饲养8 周作为对照组（con 组）；其余20
只采用高脂饲料饲养2 周，再从中选取体质量增长较快的10 只大鼠，继续高脂饮食饲养6 周，作为肥胖组（orl 组）。第8 周末，称
量两组大鼠体质量后，禁食水24 小时，次日用断尾法采血，离心分离血清，应用化学发光法检测血清雌激素（Eestrogen, E2）、卵泡
刺激素（Follicle-Stimulating Hormone, FSH）、黄体生成素（Luteinizing Hormone, LH）、睾酮（Testosterone, T）、瘦素（Leptin）及血脂水
平。结果：1.肥胖组大鼠的血清血脂Leptin 水平较对照组明显升高；2.肥胖组大鼠血清T 水平较对照组明显升高3.肥胖组大鼠血
清Leptin 水平较对照组明显升高，差异无显著性；4.肥胖组大鼠血清LH 水平较对照组稍降低，差异无显著性；5.肥胖组大鼠血清
E2、FSH 水平与对照组水平相当，无显著变化。结论：高脂饮食诱导的肥胖可能会引起大鼠一系列激素水平的变化，进而影响卵泡
发育，这可能与瘦素水平升高有关。 |
| 英文摘要: |
| Objective:To explore the influence of diet-induced obesity on follicular development of SD rats and the possible
mechanism under it, the obese rats model was set up to compare their all parameters of sexual hormones in serum and level of plasma
lipid with normal rats.Methods:10 SD female rats were randomly selected from 30 rats which were only 6 to 7 weeks, and they were
feed commonly for 8 weeks as the control group (con group); The remaining 20 rats were feed high-fat food for 2 weeks, and 10 rats of
them, whose weight increased fastest, were picked out for continuing high-fat diet for 6 weeks as obesity group (orl group). After 8
weeks, the weight of rats in both groups were measured and then, the rats were kept fasting and water-deprivation for 24 h before the
blood was taken out fromthe tail. The serumwas separated from tail blood by centrifugation and using for chemiluminescence detection
of serum E2, FSH, LH, T, and lipid levels.Results:1.The levels of serumlipid in orl group were higher than the control group obviously;
2.The levels of serum T in orl group were higher than the control group obviously; 3.The levels of serum Leptin in orl group were higher
than the control group obviously; 4. The levels of serum LH in orl group showed a decrease; 5. The levels of E2, FSH showed no significantly
change.Conclusion:There might be some influence of Diet-induced obesity may lead changes of the levels of serum sex hormone
and Leptin in SD rats, and it may influence the development of follicular. The mechanism of it is possiboly related with elevated Leptin
levels. |
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