| 李响 李郑 程燚 贾晓兰 王劲.三七总皂甙对人急性髓系白血病细胞株U937 的抑制增殖及诱导凋亡作用[J].现代生物医学进展英文版,2016,16(4):657-660. |
| 三七总皂甙对人急性髓系白血病细胞株U937 的抑制增殖及诱导凋亡作用 |
| Antiproliferative and Proapoptotic Effects of Panax Notoginsenosides onAcute Myelogenous Leukemia Cells U937 |
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| DOI： |
| 中文关键词: 三七总皂甙 细胞凋亡 白血病 Bax Bcl-2 |
| 英文关键词: Panax notoginsenosides Apoptosis Acute myelogenous leukemia Bax Bcl-2 |
| 基金项目: |
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| 中文摘要: |
| 目的：观察三七总皂甙( panax notoginsenosides，PNS )对人急性髓系白血病细胞株U937 凋亡的影响，并探讨PNS 对Bax 和
Bcl-2 蛋白表达的影响。方法：将处于对数生长期的U937 细胞分为5 组：正常对照组及PNS 组（5 mg/L、10 mg/L、20 mg/L、40
mg/L），药物作用12 h、24 h、48 h后，CCK-8 比色法检测PNS 对肿瘤细胞的相对细胞活力的影响；流式细胞术检测PNS促进细胞
凋亡的能力；RT-PCR 和Western blot 法分别检测不同浓度的PNS 对Bax 和Bcl-2 蛋白表达的影响。结果：CCK-8 分析显示，
PNS 在低浓度（≤ 40 mg/L）能明显抑制肿瘤细胞的活力，40 mg/L 处理组较正常对照组细胞活力在3 个时间点分别下降47%、
72%、85%；与对照组相比，处理组的凋亡率显著增加，10 mg/L、20 mg/L、40 mg/L 实验组凋亡率分别上升7%、10%、43%；PNS能明
显增加细胞内Bax mRNA及蛋白的表达，抑制Bcl-2 mRNA及蛋白的表达。结论：PNS 具有抑制人急性髓系白血病细胞株U937
的增殖抑制及凋亡诱导作用，并能影响Bax 和Bcl-2 蛋白的表达。文章探讨了PNS 抑制肿瘤发展可能存在的作用机制，为将来进
一步研发PNS 作为白血病治疗药物奠定基础。 |
| 英文摘要: |
| Objective:To investigate the induced-apoptosis effect of Panax notoginsenosides (PNS) in Acute myelogenous
leukemia cells U937 and the effect of PNS on Bax and Bcl-2 protein expression.Methods:Cultured-activated U937 cells were divided
into 5 groups: normal control group, PNS-treated group (5, 10, 20, 40 mg/L). The cell viability was assessed by CCK-8 Assay after 12h,
24 h and 48 h treated by PNS. After being treated with different concentrations of PNS for 24 hours, the cell apoptosis was determined by
flow cytometry. The expression of Bax and Bcl-2 mRNA and protein level were observed by RT-PCR and Western blotting, respectively.Results:PNS inhibited the growth of tumor cells in the low concentration（≤ 40 mg/L）with a dose-dependent manner. After treated by
PNS for 12 h, 24 h and 48 h, compared to the normal control group, the cell viability of 40 mg/L treated group was decreased 47%、72%、
85%, respectively. Further study on PNS showed that it induced apoptosis in U937 cell line. 10 mg/L, 20 mg/L, 40 mg/L PNS increased
the apoptosis rate by 7%, 10% and 43%, respectively, compared to that in the control group. The expression of Bax mRNA was
upregulated by PNS, wheras the mRNA level of Bcl-2 was downregulated. A Western blotting analysis indicated that PNS increased the
expression of the Bax, decreased the expression of Bcl-2.Conclusion:These results indicate that PNS exhibits significant activity against
acute myelogenous leukemia cells U937 in vitro and can induce apoptosis besides it can influence the expression of Bax and Bcl-2. PNS
is a potential therapeutic agent for acute myelogenous leukemia. |
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