| 郑艳秋 胡文良△ 崔晓波 孙学威 王亚平 孙源昊.CDC42 抑制剂ML141对喉癌Hep-2 细胞增殖的抑制作用[J].现代生物医学进展英文版,2016,16(4):644-646. |
| CDC42 抑制剂ML141对喉癌Hep-2 细胞增殖的抑制作用 |
| CDC42 Inhibitor ML141 Suppresses Laryngeal Cancer Hep-2 CellProliferation |
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| DOI： |
| 中文关键词: 喉癌 CDC42 ML141 细胞增殖 |
| 英文关键词: Laryngweal cancer CDC42 ML141 Cell proliferation |
| 基金项目:内蒙古自治区自然科学基金项目（2014MS0856）；内蒙古医科大学科技百万工程项目（KJbw2013014） |
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| 中文摘要: |
| 目的：探讨CDC42 抑制剂ML141 对喉癌细胞增殖的抑制作用，为喉癌的分子治疗提供新的靶点。方法：体外培养人喉癌
Hep-2细胞。实时定量聚合酶链反应（Real-time PCR）检测CDC42 在Hep-2细胞中的表达。利用GLISA 法检测ML141 对CDC42
活性的抑制效果。利用CCK8 法检测ML141 对Hep-2 细胞增殖能力的抑制效果。结果：①Real-time PCR 结果显示在人喉癌
Hep-2 细胞中CDC42 显著高表达（P<0.001），证明全基因组的结果准确。②GLISA 结果显示表皮生长因子作用的Hep-2细胞中
CDC42 的活性明显高表达，但加入ML141的Hep-2细胞中CDC42 的活性受到明显抑制。（P<0.001）。③CCK8 结果显示24 h，48
h和72 h时，ML141 处理的Hep-2 细胞的增殖能力与对照组相比均受到明显抑制。（P<0.001）。结论：促癌基因CDC42 抑制剂
ML141 能够抑制人喉癌Hep-2 细胞增殖，具有成为抗喉癌新药的潜力，为喉癌的分子治疗提供新的切入点。 |
| 英文摘要: |
| Objective:Investigating the effect of CDC42 inhibitor ML141 on Hep-2 cell proliferation to provide new targets for
molecular therapy of laryngeal.Methods:Hep-2 cells were cultured in vitro. Real-time PCR was used to identify the expression of
CDC42 in Hep-2 cell. GLISA was carried out to detect the changes in activation of CDC42 in Hep-2 cell treated with ML141. CCK8
assay was used to detect the effect of ML141 on Hep-2 cell proliferation.Results:① The result of Real-time PCR showed that CDC42
gene was differentially expressed in Hep-2 cells, which consistent with the results of the whole genome profiling (P<0.001). ②The result
of GLISA showed that EGF can increase the activation of CDC42 in Hep-2 cell but ML141 can inhibit its activation significantly. (p<0.
001). ③The result of CCK8 showed that the proliferation of Hep-2 cell was significantly inhibited by treating with ML141 after 24 h, 48 h
and 72 h compared with control group(P<0.001).Conclusion:CDC42 inhibitor ML141 can inhibit the proliferation of Hep-2 cell. ML141
may have the potential to become the new anti-cancer drug, which provided a new target point for molecular therapy of laryngeal cancer. |
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