| 孟祥璐 彭晓燕 逄明杰 郭菲菲 孙向荣 公衍玲 徐珞.果糖暴食对大鼠下丘脑外侧核与伏隔核Orexin 神经元的影响[J].现代生物医学进展英文版,2016,16(4):639-643. |
| 果糖暴食对大鼠下丘脑外侧核与伏隔核Orexin 神经元的影响 |
| Effect of Fructose Bingeing Behavior on Nucleus Accumbens and LateralHypothalamic Orexin Neurons of Rat |
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| DOI： |
| 中文关键词: 摄食 Orexin-A SB-334867 果糖暴食 |
| 英文关键词: Food intake Orexin-A SB-334867 Fructose bingeing |
| 基金项目:国家自然科学基金项目(81470815，31071014，81100260，81270460，81300281)；
山东省优秀中青年科学家科研奖励基金项目（BS2014YY009）；青岛市科技局项目（13-1-4-170-jch，14-2-3-3-nsh） |
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| 中文摘要: |
| 目的：探讨给予间歇性摄食大鼠模型（Intermittent Access Model [IAM]）果糖是否可使IAM 大鼠对果糖产生暴食行为，以及
下丘脑外侧核（LHA）和伏隔核Orexin（ORX）神经元对果糖暴食行为的影响。方法：给予IAM大鼠4 %、8 %或12 %的果糖溶液及
生理盐水，观察和记录大鼠果糖摄入量及能否产生果糖暴食行为；测定果糖凝集反应大鼠伏隔核壳（NAc shell）、伏隔核核（NAc
core）和背侧纹状体（Dorsal striatum）多巴胺受体（D1R、D2R）数目(Bmax)和受体亲和力(Kd)；分别监测长期和短期IAM 大鼠室旁
核（PVN）、杏仁核（CeA）、伏隔核（NAc）等相关核团的C-Fos 神经元活性（Fos-IR）；给予长期IAM 大鼠OX1R 拮抗剂SB334867，
记录大鼠摄食量。结果：长期IAM大鼠表现出果糖暴食行为，但相应的多巴胺受体数目并未改变。与对照组相比，果糖暴食组大鼠
伏隔核c-Fos 蛋白减少，神经元活性降低。短期IAM大鼠可产生果糖暴食但Fos-IR未改变。给予长期IAM大鼠OX1R拮抗剂
SB-334867（30 mg/kg），大鼠果糖暴食量和食物摄入量均减少。结论：长短期IAM大鼠均可产生果糖暴食行为；仅长期果糖暴食可
致Orexin 释放增加，减少伏隔核Orexin 神经元激活，增强外侧核Orexin 神经元激活。 |
| 英文摘要: |
| Objective:To investigate whether the rats given glucose in the intermittent access model (IAM) can cause hedonic
feeding including bingeing, and the influence of nucleus accumbens and lateral hypothalamic (LHA) orexin（ORX）neurons on fructose
eating behavior.Methods:The IAMrats were given 4 %, 8 %or 12 %fructose or Saline(NS). And the fructose intake and fructose eating
behavior were observed and recorded in the rats. The receptors number (Bmax) and receptor affinity (Kd) of dopamine D1R and D2R
were analyzed in the nucleus accumbens shell, nucleus accumbens core and dorsal striatum. The C-Fos neurons immunoreactivity
（Fos-IR）were recorded in paraventricular nucleus, amygdala, nucleus accumbens of related nucleus of the long-termand short-term IAM
rat. Food intake was observed while administration of OX1R antagonist SB334867 in the long term IAM rats.Results:The rats given
fructose solutions displayed fructose bingeing but unaltered DA D1R or D2R number following long-term exposure to the IAM. Fructose
bingeing rats, as compared to chow bingeing controls, exhibited reduced nucleus accumbens neuron activation, as determined by
c-Fos-immunoreactivity. Following short-term access to the IAM, rats exhibited bingeing but unchanged Fos-IR. Fructose bingeing and
food intake were significantly reduced while OX1R antagonist SB-334867 (30 mg/kg) were administrated for 21 days in the IAM rats.Conclusion:Long-term fructose bingeing can increase the release of Orexin, reduce the nucleus accumbens Orexin neuron activation,
enhance the lateral nucleus Orexin neurons activation. |
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