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目的：为了进一步研究苯丙胺神经毒性作用机制，我们对大鼠进行不同时长的反复苯丙胺刺激，检测大鼠部分脑区中蛋白羰基化的变化情况，我们的研究为苯丙胺的成瘾及治疗提供了新的理论依据。方法：分别对大鼠进行1 d、3 d、7 d、10 d及14 d的苯丙胺反复刺激，进行旷场测试检测其活动量变化后，采用DNPH 法检查的大鼠大脑前皮层、海马区、杏仁核三大脑区总蛋白的蛋白羰基化水平变化，探讨反复苯丙胺刺激对大鼠脑部蛋白羰基化的影响。结果：苯丙胺刺激7 d及14 d时，大鼠活动量出现了显著性增加，同时大鼠前皮层总蛋白的蛋白羰基化也出现了显著性增加，而海马区及杏仁核区域总蛋白的蛋白羰基化没有明显变化。结论：反复苯丙胺刺激能够增加大鼠活动量及大脑前皮层总蛋白蛋白羰基化水平。

英文摘要:

Objective:To further understand the mechanism of amphetamine neurotoxicity, we detected the total protein carbonylation in different regions in rats brain after repeated amphetamine stimulation, our research provide a new theoretical basis for amphetamine addiction and therapy.Methods:Rats were treated with amphetamine at 2 mg/kg daily for 1, 3, 7, 10 or 14 days. Open field test were performed to detect locomotor activity. After behavioral test, frontal cortex, hippocampus and amygdala were isolated. Total protein carbonylation were measured in these regions using DNPH method.Results:Chronic treatment with amphetamine for 7 or 14 days significantly increased the locomotor activity and the total protein carbonylation level in rat frontal cortex, but there were no difference in rat hippocampus and amygdala.Conclusion:Repeated amphetamine stimulation significantly increased the rat locomotor activity and the total protein carbonylation level in rat frontal cortex.

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