| 初银珠 徐秀梅 王腾 吉莉莉 王心宇 黄波 刘海霞.表达K1-5 蛋白的人胎盘间充质干细胞抑制大鼠主动脉环血管生成的实验研究[J].现代生物医学进展英文版,2016,16(4):616-620. |
| 表达K1-5 蛋白的人胎盘间充质干细胞抑制大鼠主动脉环血管生成的实验研究 |
| A Study on the Effects of Human Placenta-derived Mesenchymal StemCellsExpressing Kringle1-5 Protein on Angiogenesis of Aortic Ring in Rats |
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| DOI： |
| 中文关键词: 血管生成 人胎盘 间充质干细胞 Kringle1-5 动脉环 |
| 英文关键词: Angiogenesis Human placenta Mesenchymal stemcell(HPMSCs) Kringle1-5 Aortic ring |
| 基金项目:黑龙江省教育厅科学技术研究项目（12511330） |
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| 中文摘要: |
| 目的：观察表达外源性Kringle1-5(K1-5)蛋白的人胎盘组织的间充质干细胞(HPMSCs)在体外对大鼠主动脉环血管生成的影
响。方法：用胶原酶和贴壁法从人胎盘组织中提取间充质干细胞。选取感染复数MOI：50，将重组腺病毒载体rAd-K1-5 感染
HPMSCs至48 h；应用荧光显微镜观察转染效率。取8周雌性SD 大鼠的腹主动脉，建立主动脉环血管生成体外模型，6天后观察
基质胶内微血管形成的情况。结果：从人胎盘组织提取的成纤维样细胞具有贴壁生长特性，可向成骨细胞、脂肪细胞分化，证明从
胎盘提取的细胞是间充质干细胞。采用rAd-K1-5 腺病毒载体感染的HPMSCs 可分泌Kringle1-5 蛋白，表达K1-5 蛋白的转基因
HPMSCs处理的基质胶栓中管样结构的形成明显减少。结论：表达外源性Kringle1-5 蛋白的HPMSCs 能够体外抑制大鼠主动脉
环新生血管生成。 |
| 英文摘要: |
| Objective:We observed the effects of exogenous kringle 1-5 protein expressed from human placenta MSCs
（K1-5HPMSCs）on the angiogenesis of the rat aortic ring assay.Methods:Human placenta tissue-derived mesenchymal stem cells
(HPMSCs) were isolated from placenta tissue using a collagenase digestion and plastic adherence technique as described. HPMSCs were
infected with recombinate adenovirus vector Ad5F11Bs, rAd-Mock or rAd-K1-5, at a MOI of 50 for 48 h and the transfection efficiency
was evaluated by EGFP expression using a fluorescent microscope. We constructed a model of angiogenesis of the aortic ring assay in
vitro and rat aortic rings were photographed on day 6.Results:The fibroblast-like cell was obtained from human placenta tissue using a
collagenase digestion. The HPMSCs have the character of adherence and multi-differentiation, which is differentiated into osteoblast and
adipocyte cells in vitro. The above results demonstrate that the cells acquired from human placenta tissue in our experiment were human
placenta MSCs. The infected HPMSCs can exoressed kringle 1-5 protein with rAd5F11Bs adenovirus vector and the tube length were
less obviously in the infected HPMSCs group expressed K1-5 protein.Conclusion:Kringle1-5 protein expressed from HPMSCs
mediated by Ad5F11BK1-5 adenovirus vector suppressed the microvessels in the aortic rings assay which is the simple method to study
the angiogenesis. |
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