| 林琳 李阳 刘云奇 张文波 殷胜利.CTSB 对人脐静脉血管内皮细胞系增殖及凋亡的影响[J].现代生物医学进展英文版,2016,16(4):611-615. |
| CTSB 对人脐静脉血管内皮细胞系增殖及凋亡的影响 |
| Influence of CTSB on Proliferation and Apoptosis of Human Umbilical Vein Endothelial Cell |
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| DOI： |
| 中文关键词: 组织蛋白酶B 人脐静脉血管内皮细胞系 增殖 凋亡 |
| 英文关键词: Cathepsin B Human umbilical vein endothelial cell Proliferation Apoptosis |
| 基金项目: |
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| 中文摘要: |
| 目的：探索组织蛋白酶B（CTSB）对人脐静脉血管内皮细胞系（HUVEC）增殖及凋亡的影响。方法：采用细胞转染法得到慢病
毒空载体转染HU-MOCK 细胞及CTSB 过表达的HU-CTSB 细胞，与正常的HUVEC 细胞进行比较。采用MTT法检测HUVEC
增殖情况；流式细胞仪检测各组细胞凋亡情况；采用蛋白免疫印迹法检测Bal-2/ Bax 及Caspase 家族（Caspase-3/9）的表达情况。结
果：HU-CTSB组各时间点HUVEC 的增殖率明显低于其他两组，且差异具有统计学意义（P<0.05）；HU-CTSB组的HUVEC 早期
凋亡率（右下象限）和晚期凋亡率（右上象限）显著高于HUVEC组和HU-MOCK 组，且组间差异具有统计学意义（P<0.05）；
HU-CTSB组的抑制凋亡蛋白Bcl-2表达量明显低于其他两组，且促凋亡蛋白Bax表达量显著高于其他两组，差异均具有统计学
意义（P<0.05）；HU-CTSB 组caspases-3 和caspases-9 的活化片段显著高于其他两组，差异均具有统计学意义（P<0.05）。结论：
CSTB 通过激活Caspase-3/9 信号通路来上调Bax，并下调Bal-2，显著抑制HUVEC的增殖，并促进其凋亡，进而影响心血管疾病
的发展。 |
| 英文摘要: |
| Objective:To explore the influence of cathepsin B (CTSB) on proliferation and apoptosis of human umbilical vein
endothelial cell (HUVEC).Methods:Empty lentivirus vector transfection HU-MOCK cells and HU-CTSB cells from CTSB over
expression cells were obtained by cell transfection method, which were compared with normal HUVEC cells. The HUVEC proliferation
status was detected by MTT method; the cell apoptosis status was detected by flow cytometry instrument; and the expressions of
Bal-2/Bax and Caspase-3/9 were detected by protein immunoblot method.Results:The HUVEC proliferation rate of HU-CTSB group
was significantly lower than that of the other two groups at each time point, the differences were statistically significant (P<0.05). The
HUVEC early apoptosis rate (lower right quadrant) and late apoptosis rate (upper right quadrant) of HU-CTSB group were significantly
higher than those of HUVEC and HU-MOCK groups, the differences were statistically significant (P<0.05). The expression of inhibiting
apoptosis protein Bcl-2 in HU-CTSB group was significantly lower than that of the other two groups, while the expression of promoting
apoptosis proteins Bax in HU-CTSB group was significantly higher than that of the other two groups, the differences were statistically
significant (P<0.05). The activation pieces of caspases-3 and caspases-9 of HU-CTSB group were significantly higher than the other two
groups, the differences were statistically significant (P<0.05).Conclusion:CTSB can raise Bax and cut down Bal-2 by activating the
signaling pathways of Caspase-3/9, which will remarkably inhibit the proliferation, promote the apoptosis of HUVEC, and influence the
development of cardiovascular disease. |
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