| 马超 刘雨桐 贾利云 梁桐 张淑平△.晶状体上皮源性生长因子对MGARP基因的调控作用研究[J].现代生物医学进展英文版,2016,16(4):601-605. |
| 晶状体上皮源性生长因子对MGARP基因的调控作用研究 |
| Study on the Regulation of LEDGF to the MGARP Gene |
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| DOI： |
| 中文关键词: MGARP LEDGF/p75 Sp1 基因调控 |
| 英文关键词: MGARP LEDGF/p75 Sp1 Gene Regulation |
| 基金项目:国家自然科学基金项目（81171063） |
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| 中文摘要: |
| 目的：研究HEK-293T 细胞中晶状体上皮源性生长因子(lens epithelium-derived growth factor, LEDGF) 对富含酸性氨基酸的
线粒体定位蛋白（Mitochondria-localized Glutamic Acid Rich Protein,MGARP ）基因表达的调控作用。方法：将不同剂量的含有LEDGF
基因的载体，转入HEK-293T 细胞中，通过Western blot 和启动子报告分析检测的方法，检测细胞内MGARP的表达水
平。并采用LEDGF和sp1共同转染的方法，来检测二者对MGARP转录活性的协同调控作用。结果：与空载体对照组相比，随着
含有LEDGF基因载体剂量的增加，HEK-293T细胞内MGARP的表达也明显上调（P<0.05），同时共同转染LEDGF和Sp1后，细
胞内的MGARP转录活性比单独过量表达LEDGF或者Sp1的转录活性明显增高（P <0.05）。结论：在HEK-293T 细胞中，LEDGF
可以调控的MGARP表达，而且， LEDGF和Sp1对MGARP的调控具有协同作用。 |
| 英文摘要: |
| Objective:To study the role of lens epithelium-derived growth factor (LEDGF ) in regulating Mitochondria-localized
Glutamic Acid Rich Protein ( abbreviated as MGARP ) in HEK-293T cells.Methods:The different dosages of LEDGF were transfected
into the HEK-293T cells, which was followed by Western blot and promoter report to detect the expression of MGARP in HEK-293T
cell. Furthermore, we co-transfected Spa with LEDGF into cells to test their synergistic regulation to the transcriptional activity of MGARP promoter.Results:As the increase of LEDGF dosages, the expression ofMGARP was significantly upregulated compared with the control(P<0.05), and after co-transfection of Sp1 with LEDGF, the promoter activity of MGARP was remarkably enhanced and much higher than that transfected with either LEDGF or Sp1 individually（P<0.05）.Conclusion:In HEK-293T cells, LEDGF can up-regulate the expression of MGARP gene and such regulation can be synergistically enhanced by Sp1. |
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