| 杨树萌 张浩鹏 畅建平 陶宣晨 杨拓耘 杨艳梅 张新宇.CHOP在三氧化二砷诱导SMMC-7721 凋亡中的作用[J].现代生物医学进展英文版,2016,16(2):267-270. |
| CHOP在三氧化二砷诱导SMMC-7721 凋亡中的作用 |
| Role of CHOP in Arsenic Trioxide Induced SMMC-7721 Apoptosis |
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| DOI： |
| 中文关键词: 三氧化二砷 肝癌 内质网应激 CHOP |
| 英文关键词: Arsenic trioxide Hepatocellular carcinoma Endoplasmic reticulumstress CHOP |
| 基金项目:黑龙江省自然科学基金项目（D2007-79） |
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| 中文摘要: |
| 探讨CHOP 在三氧化二砷（Arsenic Trioxide，As2O3）诱导肝癌细胞系SMMC-7721 凋亡中的作用。方法：通过MTT法观
察不同浓度的As2O3对肝癌细胞系SMMC-7721 增殖活性的影响；通过流式细胞术检测不同浓度的As2O3作用后，SMMC-7721 细
胞的凋亡率；以Western-blot方法检测As2O3处理SMMC-7721 细胞后，内质网应激相关蛋白GRP78，CHOP 的变化。通过CHOP
siRNA沉默CHOP 后，观察As2O3对SMMC-7721细胞凋亡的影响。结果：As2O3能显著抑制SMMC-7721 细胞的增殖活性，并呈
剂量依赖性诱导SMMC-7721细胞的凋亡；Western blot 结果显示三氧化二砷诱导内质网应激相关蛋白的表达。CHOP siRNA 沉
默CHOP 后能够显著抑制As2O3对SMMC-7721 细胞凋亡的诱导。结论：As2O3诱导SMMC-7721细胞凋亡可能与其诱导CHOP
表达有关。 |
| 英文摘要: |
| Objective:To investigate the effect of CHOP on arsenic trioxide (As2O3) induced hepatoma carcinoma cell line
SMMC-7721 apoptosis.Methods:The effect of different concentrations of As2O3 on the proliferation of SMMC-7721 was detected by
MTT. The apoptosis rate of SMMC-7721 cells was detected by flow cytometry after the treatment of different concentrations of As2O3.
ER stress related protein GRP78 and CHOP expression in SMMC-7721 cell was detected by Western-blot after the treatment of As2O3.
The effects of As2O3 on apoptosis of SMMC-7721 cell after CHOP silenced was detected by CHOP siRNA.Results:As2O3 could
significantly inhibit the proliferation of SMMC-7721 cell and it could dose-dependently induce SMMC-7721 cell apoptosis (P＜0.05).
Western-blot results showed the expression of endoplasmic reticulum stress related proteins GRP78 and CHOP had been changed in
SMMC-7721 cell with the treatment of arsenic trioxide (P＜0.05).Conclusion:The apoptosis induction of SMMC-7721 cell by As2O3
may be related to the induction of CHOP. |
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