| 徐仑 聂嘉 陈冬冬 孙鹏 刘斌 李岩峰.两种Wnt 通路抑制剂对牙周膜干细胞作用的比较研究[J].现代生物医学进展英文版,2016,16(2):252-256. |
| 两种Wnt 通路抑制剂对牙周膜干细胞作用的比较研究 |
| Comparative Research on the Effect of two Wnt Signaling Inhibitor on the Proliferation and Osteogenic Differentiation of PDLSC |
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| DOI： |
| 中文关键词: 牙周膜干细胞 XAV-939 DKK1 Wnt 信号通路 |
| 英文关键词: PDLSC XAV-939 DKK1 Wnt passway |
| 基金项目:首都卫生发展科研专项（首发2014-4-5022）；解放军总医院临床扶持基金（2014FC-SXYY-1003）；保健专项课题资助（14BJZ03） |
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| 中文摘要: |
| 目的：本实验主要探讨Wnt通路抑制剂XAV-939 相比DKK1在牙周膜干细胞增殖及矿化中的作用差异。方法：酶消化法培
养牙周膜干细胞，鉴定后，用CCK8 试剂盒检测XAV-939 和DKK1 对牙周膜干细胞增殖能力的影响，茜素红染色及定量检测
XAV-939 和DKK1 对牙周膜干细胞成骨分化能力的影响，qRT-PCR检测DKK1 和XAV-939 对牙周膜干细胞Wnt通路相关基因
GSK-3茁和茁-catenin 及成骨分化相关基因ALP，DSPP，BSP, OCN, RUNX-2 的影响。结果：XAV-939 和DKK1 都可以通过抑制
Wnt 通路来抑制牙周膜干细胞的增殖及成骨分化。当没有外源性Wnt蛋白刺激时，XAV-939 作为Wnt通路抑制剂的抑制作用要
强于DKK1，而加入外源性Wnt 蛋白后，XAV-939 与DKK1 的作用效果相当。结论：XAV-939 对比DKK1，具有更为广泛而稳定
的抑制效果。XAV-939 可以作为高效的Wnt通路抑制剂应用于未来关于牙周膜干细胞和Wnt 通路相关实验研究中。 |
| 英文摘要: |
| Objective:To evaluate the effect of Wnt inhibitor XAV-939 on the proliferation and osteogenic differentiation of
PDLSC and DKK1.Methods:PDLSCs were isolated from healthy parodontium of premolar teeth of individuals undergoing tooth
extraction for orthodontic treatment. After identification, we used cck8 kit to evaluate the effect of XAV-939 and DKK1 on the
proliferation of PDLSC. Alizarin Red Staining and quantification was used to evaluate the effect of XAV-939 and DKK1 on the
osteogenic differentiation of PDLSC. The effect of XAV-939 and DKK1 on the gene expression of PDLSC, including Wnt signaling
marker genes GSK-3beta, beta-catenin and osteogenic differentiation marker genes ALP, DSPP, BSP, OCN, RUNX-2 was evaluated by
qRT-PCR.Results:XAV-939 and DKK1 could inhibit the proliferation and osteogenic differentiation of PDLSC by inhibiting Wnt
passway. The inhibiting effect of XAV-939 was stronger than DKK1 when there wasn't exogenous Wnt protein. And there weren't
statistical differences between XAV-939 and DKK1 after the addition of exogenous Wnt protein.Conclusion:The effect of XAV-939
was more extensive and stable. XAV-939 can be used as a high-efficiency Wnt inhibitor in the future research on PDLSC and Wnt
passway. |
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