| 邹臣丹 吴明娟 李强 王小娜 程万鹏 张赫 邹朝霞 高旭.microRNA-125b 抑制结肠癌细胞增殖的研究[J].现代生物医学进展英文版,2016,16(2):238-241. |
| microRNA-125b 抑制结肠癌细胞增殖的研究 |
| miR-125b Suppresses the Proliferation of Colon Cancer Cells |
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| DOI： |
| 中文关键词: 结肠癌 微小RNA 增殖 裸鼠 动物模型 |
| 英文关键词: Colon cancer MicroRNA Proliferation Nude mice Animal models
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| 基金项目:黑龙江省教育厅课题（12531313）；黑龙江省自然科学基金青年基金项目（QC2001C016） |
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| 中文摘要: |
| 目的：探究在多种肿瘤中均发挥重要调节作用的miR-125b 对结肠癌细胞增殖的影响。方法：采用miR-125b 过表达的结肠癌
细胞系HCT116，在细胞水平验证miR-125b 对细胞增殖能力的影响。采用皮下注射的方式，建立裸鼠结肠癌皮下移植瘤模型，以
研究miR-125b 对结肠癌体内增殖的影响。在临床样本组织中检测miR-125b 的表达以佐证miR-125b 功能。结果：在体外MTT 实
验中，miR-125b 能够抑制结肠癌细胞系HCT116 的增殖（P<0.001）。在体内模型中，注射过表达miR-125b mimics的HCT116 细胞
所形成的肿瘤与对照组相比，生长速度缓慢，肿瘤大小亦明显低于对照组（P<0.05）。在临床样本中对比癌组织与癌旁正常组织中
miR-125b 的表达量，发现miR-125b 在癌旁组织中表达明显高于癌组织。结论：miR-125b 能够抑制结肠癌细胞的增殖。 |
| 英文摘要: |
| Objective:To investigate the role of miR-125b in the cell proliferation of colon cancer.Methods:The
miR-125b-overexpressed HCT116 cells were used to test the effects of miR-125b on the proliferative ability of cells. Then the above cells
were used to subcutaneous injection to establish the nude mice xenograft model. The growth of the tumors was detected for valuating the
effect of miR-125b on colon cancer cells. The expression of miR-125b was also tested in clinical tissue.Results:According to the results
of MTT, miR-125b could repress the proliferation of HCT116 （(P<0.001). In vivo, compared with the control group, the growth of
xenograft tumors of over-expressed miR-125b was significantly slower and the volume was smaller (P<0.05). The expression of
miR-125b in cancer tissue was much lower compared with normal mucosa tissue adjacent to cancer (P<0.05).Conclusion:miR-125b is
a kind of anti-proliferative microRNA in colon cancer. |
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