| 孙书红 孟宏涛 惠增骞 马兰香 原平利 王顺.n-3PUFA对高脂SHR 大鼠血压及血浆游离脂肪酸谱的影响[J].现代生物医学进展英文版,2016,16(2):225-229. |
| n-3PUFA对高脂SHR 大鼠血压及血浆游离脂肪酸谱的影响 |
| Effect of the n-3 Fatty Acids on Blood Pressure and SerumFree Fatty AcidElements in SHR Rats under High-fat Diet |
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| DOI： |
| 中文关键词: 脂肪酸 自发性高血压大鼠 高血压 |
| 英文关键词: Fatty acids Spontaneously Hypertensive Rats Hypertension |
| 基金项目:国家自然科学基金项目(30800472) |
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| 中文摘要: |
| 目的：观察高饱和脂肪酸及n-3 多不饱和脂肪酸饮食后对自发性高血压大鼠血压、静息心率、体重、血脂、血糖及游离脂肪酸
谱的影响。方法：选择8 周龄雄性自发性高血压大鼠（SHR）30 只和同龄对照大鼠（WKY）30 只，随机分为6 组：SHR、WKY 普通
饲料组各10 只，SHR、WKY 高脂组各10 只，SHR、WKY高脂加鱼油饮食组各10 只，持续喂养至16 周龄。干预期间每两周测定
血压和体重，干预前后测定静息心率、血脂、血糖及血浆游离脂肪酸谱。结果：（1）血压和静息心率的变化：SHR 大鼠高脂饮食组较
普食组血压水平显著性增高，而高脂加鱼油饮食组较高脂饮食组血压水平显著性减低；WKY 大鼠高脂饮食组较普食组血压水平
显著性增高，而高脂加鱼油饮食组较高脂饮食组血压水平显著性减低；SHR大鼠高脂饮食组较普食组静息心率显著性增高（P=0.
007），而高脂加鱼油饮食组较高脂饮食组静息心率有下降趋势，但差异无显著性（P=0.125），WKY 大鼠静息心率各组间无明显差
异。（2）血浆游离脂肪酸谱：与WKY 大鼠比较，SHR 大鼠中亚麻酸（Linolenic acid, ALA）、花生四烯酸（Linoleic Acid, AA）与n-6
多不饱和脂肪酸（n - 6 polyunsaturated fatty acids, n-6PUFA 含量增高，高脂饮食增加了饱和脂肪酸（Saturated fatty acid, SFA），有显
著差异（P<0.05），高脂鱼油组二十二碳六烯酸（Docosahexaenoic acid, DHA）及二十碳五烯酸（Docosapentaenoic acid, EPA）增加导
致n-3 多不饱和脂肪酸（n - 3 polyunsaturated fatty acids, n-3PUFA）含量增加（P<0.05），SHR大鼠高脂鱼油组亚油酸（Linoleic Acid,
LA）、AA含量减低（P<0.05）。结论：膳食补充n-3PUFA 可能通过影响交感神经活性和血浆脂肪酸谱的组成而改善高饱和脂肪酸
所致SHR 大鼠的血压升高。 |
| 英文摘要: |
| Objective:To investigate Saturated fatty acids and n-Polyunsaturated fatty acid of dietary intervention on blood
pressure, rest heart rate, weight, blood lipids, blood glucose and free fatty acid composition（FFA）in the spontaneously hypertensive rat
model.Methods:30 male spontaneously hypertensive rats (SHR) of 8 weeks old were randomly divided into 3 groups: normal fat group
[SHR-CON], high-fat diet group[SHR+HF] and high-fat diet group supplemented with fish oil [SHR+HF+FOD]. 30 male Wistar-Kyoto
rats (WKY)at the same age were served as normal controls，divided into 3 groups (normal rat chow group [WKY+CON] , high-fat diet
group [WKY+HF] and high-fat diet group supplemented with fish oil [WKY+HF+FOD] ). Then these rats were sacrificed randomly at
16th week of this study，blood pressure and weight were measured every two weeks. Plasma was isolated and Plasma glucose, lipid levels
were measured with automatic biochemical apparatus. Plasma FFA was measured by high-performance liquid chromatogram using direct
derivatization method.Results:(1) BP raised significantly in SHR than the in WKY . In the HFD group of SHR, BP was raised markedly
at 16 weeks of age. In contrast, the BP of the HF+FOD group gradually decreased to a level as low as the one in the control group. The
supplementation with fish oil resulted in blood pressure reduction in both SHR and WKY. In the HFD of SHR, HR increased significantly
in resting periods. A slight decrease could even be observed in the HF+FOD of SHR during the resting period. All of the rats displayed a
very similar resting heart rate in WKY. (2)SHR, as compared to WKY, showed higher ALA, AA and n-6PUFA levels on all diets. In SHR
fed HF diet, the levels of SFA（PA, SA）were higher .The content of n-3PUFA increased, ALA and AA declined in the HF+FOD groups
of SHR and WKY.Conclusion:The results indicate that a increase in the daily intake of n-3 PUFA lowers BP of SHR depending on its
intrinsic sympathomimetic activity and fatty acid composition. |
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