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目的：研究急性一氧化碳中毒后大鼠海马组织NaPi-IIb 蛋白的表达情况。方法：选取雄性Sprague-Dawley（SD）大鼠48 只，随机分为正常对照组（NC 组）14 只、缺氧组（IB 组）17 只、急性CO 中毒组（CO-P 组）17 只。利用简易密闭容器建立缺氧模型；利用多次追加腹腔注射CO气体染毒的方法建立急性一氧化碳中毒模型。观察各组大鼠在建模过程中呼吸、活动改变情况。大鼠于建模24 h-26 h后取脑组织制作石蜡切片，选取海马组织平面利用免疫组织化学染色（IHC）观察组织形态及海马组织中NaPi-IIb 蛋白的表达情况。同时，分离各组大鼠海马组织，利用蛋白质免疫印迹技术（Western Blot，WB）检测海马组织中NaPi-IIb 蛋白的表达情况。结果：IHC、WB结果表明海马组织中NaPi-IIb 蛋白的表达在CO-P组中明显高于NC 组、IB 组（P<0.05）。NC组、IB 组比较， NaPi-IIb蛋白的表达无明显差异（P＞0.05）。结论：在本研究中，CO暴露后，大鼠海马组织NaPi-IIb 蛋白表达的增高与CO相关。 NaPi-IIb蛋白可能成为CO中毒稳定、可靠的诊断标志物。

英文摘要:

Objective:To study the expression of NaPi-IIb in rat hippocampus after acute carbon monoxide poisoning.Methods:Forty-eight male SD rats were randomly divided into NC group(n=14), IB group(n=17) and CO-P group(n=17). We used simple sealed containers to establish atmospheric model of acute hypoxia and established the acute carbon monoxide (CO) poisoning model by the method of several additional intraperitoneal injection of 99.99 %CO gas. The changes in breathing, behavior of rats were observed in IB group and CO-P group. The brain of rats was produced paraffin after 24 h-26 h, immunohistochemical (IHC) technique was used to observe the histological changes of hippocampus and Western blot (WB) was used to detect the expression of NaPi-IIb in rat hippocampus.Results:IHC and WB results showed that the expression of NaPi-IIb in hippocampus in CO-P group was significantly higher than that in NC group and IB group (P<0.05). The expression of NaPi-IIb was no significant difference between NC group and IB group (P＞0.05).Conclusion:In this research,the increase of the expression of NaPi-IIb was associated with CO. NaPi-IIb may be the stable and reliable diagnostic marker of carbon monoxide poisoning.

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