| 李淑媛 邢焱玲 孙敬霞 吴春凤 何秀萍.子痫前期患者血清对脐静脉内皮细胞CD40 和NF-kB 表达的影响[J].现代生物医学进展英文版,2016,16(1):117-120. |
| 子痫前期患者血清对脐静脉内皮细胞CD40 和NF-kB 表达的影响 |
| Influence of Preeclampsia Patients' Serumon Human Umbilical VeinEndothelial Cell CD40 and NF-kB Expression |
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| DOI： |
| 中文关键词: 子痫前期 人脐静脉内皮细胞 核因子-kB CD40 |
| 英文关键词: Preeclampsia Human Umbilical Vein Endothelial Cells(HUVEC) Nuclear factor- kappaB(NF-kB) CD40 |
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| 中文摘要: |
| 目的：研究CD40 和NF-资B 在子痫前期患者组的脐静脉内皮细胞（HUVEC）中的表达，探讨其在子痫前期发病过程中的作
用。方法：应用子痫前期患者组的血清冲击人HUVEC 株，电镜下观察HUVEC 的形态变化，流式细胞仪下测定细胞的凋亡，用
RT-PCR 方法检测HUVEC中CD40 和NF-资B 的表达情况。结果：①电镜下正常孕妇组HUVEC呈" 铺路石" 状单层镶嵌排列，
子痫前期组HUVEC形态呈典型损伤改变，细胞稀疏，细胞核与细胞浆分界模糊不清，胞浆中有暗色颗粒。②流式细胞仪测定子
痫前期组HUVEC的细胞凋亡较正常组明显升高，差异有显著意义（P<0.05）；③ RT-PCR 测定显示子痫前期患者组HUVEC 中
CD40mRNA 及NF- BmRNA 水平均较正常组明显升高，差异有显著意义（P<0.05）；④ 子痫前期组及对照组的CD40 和NF- kB表
达均呈显著性正相关。结论：CD40和NF-资B 的激活均参与了子痫前期的发病机制，并在其中发挥重要作用。 |
| 英文摘要: |
| Objective:To study expression of CD40 and NF-kB in preeclampsia patients' human umbilical vein endothelial cell
(HUVEC) and discuss its effect in the process of preeclampsia attack.Methods:Preeclampsia patients' serumwas used to impact people's
HUVEC strains, electron microscopy was applied to observe changes in HUVEC, flow cytometry was utilized to measurecell apoptosis,
RT-PCR was adopted to detect expression of CD40 and NF-kB in HUVEC.Results:①Under the electron microscopy, cells in normal
pregnant women group were in single mosaic array in the formation of "paved road stone", but the preeclampsia group's HUVEC cellular
morphology had typical damage changes, cells were sparse, the boundary between cytoplasm and cell nucleus was unclear and dark color
particles existed in cytoplasm; ② The preeclampsia group's HUVEC cell apoptosis was obviously improved compared with the normal
group and difference had obvious significance P<0.05; ③RT-PCR showed that level of CD40mRNA and NF-kBmRNA in preeclampsia
group's HUVEC was obviously improved compared with the normal group and difference had obvious significance P<0.05; ④ CD40 and
NF-kB expression of the preeclampsia group and the control group had significant positive correlation.Conclusion:Activation of CD40
and NF-kB took part in pathogenesis of preeclampsia and played an important role in it. |
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