| 史承勇 周磊 杜贺 郭志福 李峰 陈少萍.17-AAG 对颈动脉球囊损伤大鼠血管平滑肌细胞增殖影响的实验研究[J].现代生物医学进展英文版,2015,15(36):7054-7057. |
| 17-AAG 对颈动脉球囊损伤大鼠血管平滑肌细胞增殖影响的实验研究 |
| Study of the Influence of 17-Allylamino-17-emethoxy-geldanamycin to the Proliferation of Vascular Smooth Muscle Cells after Carotid Artery BalloonInjury in Rats |
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| DOI： |
| 中文关键词: 17-AAG 血管平滑肌细胞 颈动脉球囊损伤 增殖 内膜增生 |
| 英文关键词: 17-AAG Vascular smooth muscle cell Carotid artery balloon injury Proliferation Intimal hyperplasia |
| 基金项目: |
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| 中文摘要: |
| 目的：研究17- 丙烯胺-17 去甲氧格尔德霉素（17-Allylamino-17-emethoxy-geldanamycin, 17-AAG）对球囊损伤后大鼠颈动脉
血管平滑肌细胞增殖的影响。方法：选取雄性Sprague-Dawley（SD）大鼠30 只，随机数字法分为球囊损伤（Balloon injury，BI）组10
只、球囊损伤+17-AAG 低剂量治疗（Balloon injury+Low dose AGG，BIL）组10 只、球囊损伤+17-AAG 高剂量治疗（Balloon
injury+High dose AGG，BIH）组10只。建立大鼠颈动脉球囊损伤模型，病理学评估血管内膜增生情况。各组于球囊损伤28 天后取
材，将损伤区域的血管段取材固定，苏木精- 伊红染色（HE）观察血管并测量内膜面积（Intimal Area, IA）、中膜面积（Membrane
Area,MA），计算内膜/中膜面积比（IA/MA），以评估内膜增生情况；同时，利用免疫荧光染色（Immunofluorescence staining, IFS）观
察增殖细胞核抗原（Proliferating cell nuclear antigen, PCNA）表达的情况，以评价血管平滑肌细胞的增殖情况。结果：大鼠颈动脉球
囊损伤模型成功建立。HE 染色后计算血管I/M比值，结果表明BIL组与BI组血管I/M 比值无统计学差异（P>0.05），BIH 与BI组
血管I/M比值有统计学差异（P<0.05）。IFS结果表明，BIL组血管壁PCNA 表达较BI组略有降低，但无统计学意义（P>0.05）。BIH
组血管壁PCNA表达较BI组明显减低，有显著统计学差异（P<0.05）。结论：一定浓度的17-AAG可明显的抑制球囊损伤后颈动脉
血管平滑肌细胞增殖；17-AAG可以成为球囊损伤后大鼠血管平滑肌细胞增殖的抑制剂。 |
| 英文摘要: |
| Objective:To study the influence of 17-Allylamino-17-emethoxy-geldanamycin (17-AAG) to the proliferation of rat
vascular smooth muscle cells after carotid artery balloon injury.Methods:Thirty male SD rats were divided into BI group (n=10), BIL
group (n=10) and BIH group (n=10) randomly. We established carotid artery balloon injury model of rats and assessed the proliferation of
intimal hyperplasia by pathology. After carotid artery balloon injury in 28 days, the damage area of the segment were fixed and taken to
HE staining for measuring intimal area (IA), membrane area (MA) and IA/MA ratio. Then observed the expression of proliferating cell
nuclear antigen (PCNA) by immunofluorescence staining to evaluate the proliferation of vascular smooth muscle cells.Results:The
carotid artery balloon injury model of rats were successfully established. The results of HE staining showed that I/M ratio of BIH group
had significantly difference with that of BI group (P<0.05). I/Mratio was no significant difference between BI group and BIL group (P＞
0.05). The results of immunofluorescence staining showed that the expression of PCNA in BIL group was lower than that in BI group
(P＞0.05). The expression of PCNA in BIH group was significant lower than that in BI group (P<0.05).Conclusion:The proliferation of
vascular smooth muscle cells could be significantly inhibited by certain concentration of 17-AAG. 17-AAG could become proliferation
inhibitors to vascular smooth muscle cells after balloon injury in rats. |
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