| 孙玉敏 许晓琳 程才 杨怡 张丽萍 刘秀丽 王路 黄冬冬 吴晓华.葛根素对体外培养的人老年女性骨质疏松症成骨细胞分化的初步研究[J].现代生物医学进展英文版,2015,15(34):6649-6652. |
| 葛根素对体外培养的人老年女性骨质疏松症成骨细胞分化的初步研究 |
| Influence of Puerarin on the Differentiation of Osteoblasts in Patients withSenile Women with Osteoporosis |
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| DOI： |
| 中文关键词: 葛根素 骨质疏松症 人成骨细胞 分化 |
| 英文关键词: Puerarin Osteoporosis Human osteoblast Differentiation |
| 基金项目:河北省科学技术研究与发展计划项目（12277752）；河北省教育厅资助科研项目（Z2012113） |
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| 中文摘要: |
| 目的：观察葛根素对体外培养老年女性骨质疏松症骨折患者体外分离培养的成骨细胞分化的影响。方法：采集老年女性骨质
疏松症股骨颈骨折行人工股骨头置换术中取下的股骨颈松质骨，采用骨组织块法原代培养成骨细胞。观察不同浓度（0、0.01、0.1、
1 umol/L）葛根素对成骨细胞表达碱性磷酸酶（alkaline phosphatase, ALP）、骨钙素（osteocalcin, OC）的影响，并比较各组成骨细胞
钙化能力的大小。结果：0.01～1.00 umol/L 的葛根素随着浓度增加，成骨细胞表达ALP、OC活性呈增强趋势，各浓度组两两比较
差异均有统计学意义（P＜ 0.05）；随葛根素浓度增高，成骨细胞钙化结节数量也明显增加，组间比较差异有统计学意义（P＜ 0.05）。
结论：葛根素呈浓度依赖性促进成骨细胞表达ALP、OC并能提高成骨细胞的钙化能力。 |
| 英文摘要: |
| Objective:To observe the influence of puerarin on the differentiation of osteoblasts in patients with senile women with
osteoporosis in vitro.Methods:The cancellous bone of elderly women with osteoporosis artificial femoral head replacement patients was
collected. The osteoblasts were cultured by bone tissue block method.To observe ALP and OC expressed by osteoblasts with different
concentrations (0, 0.01, 0.1, 1 mol/L) puerarin.Results:With the increase of the puerarin concentration(0.01~1 mol/L), the expression
of ALP and OC in osteoblasts were increased, the concentration of two groups were statistically significant (P<0.05). With the increase of
the concentration of puerarin, osteoblast numbers of calcified nodules increased, there were significant differences between the groups
(P<0.05).Conclusion:0.01, 0.1, 1 mol/L puerarin dose dependently stimulated osteoblasts express ALP and OC, and can improve the
calcification capacity of osteoblasts. |
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